An enhancer responsible for activating transcription at the midblastula transition in Xenopus development

Paul A Krieg, D. A. Melton

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Transcripts from the Xenopus gene GS17 are absent from oocytes and first appear at the midblastula transition (MBT), about 8 hr after fertilization. Injection experiments with deletions of the GS17 gene and GS17-β-globin hybrid genes show that a relatively short sequence of 74 base pairs is sufficient to activate, in cis, transcription at the MBT. This 74-base sequence is located about 700 bases upstream of the GS17 promoter and has the properties of an enhancer element.

Original languageEnglish (US)
Pages (from-to)2331-2335
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume84
Issue number8
StatePublished - 1987
Externally publishedYes

Fingerprint

Xenopus
Globins
Gene Deletion
Fertilization
Base Pairing
Genes
Oocytes
Injections

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

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abstract = "Transcripts from the Xenopus gene GS17 are absent from oocytes and first appear at the midblastula transition (MBT), about 8 hr after fertilization. Injection experiments with deletions of the GS17 gene and GS17-β-globin hybrid genes show that a relatively short sequence of 74 base pairs is sufficient to activate, in cis, transcription at the MBT. This 74-base sequence is located about 700 bases upstream of the GS17 promoter and has the properties of an enhancer element.",
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AB - Transcripts from the Xenopus gene GS17 are absent from oocytes and first appear at the midblastula transition (MBT), about 8 hr after fertilization. Injection experiments with deletions of the GS17 gene and GS17-β-globin hybrid genes show that a relatively short sequence of 74 base pairs is sufficient to activate, in cis, transcription at the MBT. This 74-base sequence is located about 700 bases upstream of the GS17 promoter and has the properties of an enhancer element.

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