An extended X-ray absorption fine structure investigation of the structure of the active site of lactoperoxidase

C. S. Chang, R. Sinclair, S. Khalid, I. Yamazaki, S. Nakamura, Linda S Powers

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18 Citations (Scopus)

Abstract

Native lactoperoxidase, compound III, and the reduced forms (at pH 6 and 9) were studied using X-ray absorption spectroscopy (XAS). Native lactoperoxidase has four pyrrole nitrogen ligands at an average distance of 2.04 ± 0.01 Å, a proximal ligand at 1.91 ± 0.02 Å, and a sixth (distal) ligand at 2.16 ± 0.03 Å. Lactoperoxidase native enzyme has a first coordination shell structure that is similar to that of native lignin peroxidase [Sinclair, R., Yamazaki, I., Bumpus, J., Brock, B., Chang, C.-S., Albo, A., & Powers, L. (1992) Biochemistry 31, 4892-4900] and different from that of horseradish peroxidase [Chance, B., Powers, L., Ching, Y., Poulos, T., Schonbaum, G., Yamazaki, I., & Paul, K. (1984) Arch. Biochem. Biophys. 235, 596-611]. Similarly, lactoperoxidase compound III resembles lignin peroxidase compound III. The five-coordinated ferrous form was stable at pH 9, but at pH 6 it was rapidly converted to the six-coordinated form with a distal ligand at 2.18 ± 0.03 Å. No evidence typical of changes in spin state was obtained at the different pH values.

Original languageEnglish (US)
Pages (from-to)2780-2786
Number of pages7
JournalBiochemistry
Volume32
Issue number11
StatePublished - 1993
Externally publishedYes

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Lactoperoxidase
X ray absorption
Catalytic Domain
X-Rays
Ligands
X-Ray Absorption Spectroscopy
Biochemistry
Pyrroles
X ray absorption spectroscopy
Arches
Horseradish Peroxidase
Nitrogen
Enzymes
lignin peroxidase

ASJC Scopus subject areas

  • Biochemistry

Cite this

Chang, C. S., Sinclair, R., Khalid, S., Yamazaki, I., Nakamura, S., & Powers, L. S. (1993). An extended X-ray absorption fine structure investigation of the structure of the active site of lactoperoxidase. Biochemistry, 32(11), 2780-2786.

An extended X-ray absorption fine structure investigation of the structure of the active site of lactoperoxidase. / Chang, C. S.; Sinclair, R.; Khalid, S.; Yamazaki, I.; Nakamura, S.; Powers, Linda S.

In: Biochemistry, Vol. 32, No. 11, 1993, p. 2780-2786.

Research output: Contribution to journalArticle

Chang, CS, Sinclair, R, Khalid, S, Yamazaki, I, Nakamura, S & Powers, LS 1993, 'An extended X-ray absorption fine structure investigation of the structure of the active site of lactoperoxidase', Biochemistry, vol. 32, no. 11, pp. 2780-2786.
Chang, C. S. ; Sinclair, R. ; Khalid, S. ; Yamazaki, I. ; Nakamura, S. ; Powers, Linda S. / An extended X-ray absorption fine structure investigation of the structure of the active site of lactoperoxidase. In: Biochemistry. 1993 ; Vol. 32, No. 11. pp. 2780-2786.
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