An HPLC method for quantitation of perillyl alcohol in a topical pharmaceutical cream formulation

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11 Citations (Scopus)

Abstract

A reverse phase high performance liquid chromatographic method for quantitation of perillyl alcohol in a topical cream pharmaceutical formulation was developed. Previously reported methods for analyzing drugs in lipid formulations are relatively complex and time consuming, with extraction, purification and derivatization involved. Through a simple dilution of the cream formulation in isopropyl alcohol, the present assay method enables the direct injection of the samples, on an Alltima C18 5 μ, 150 mm × 2.1 mm, narrow bore column (Alltech Associates, Deerfield, IL). The method includes an isocratic run with acetonitrile-water (40:60, v/v) at 0.35 mL/min for 12 min, followed by a gradient wash with isopropyl alcohol for 20 min, to ensure that all formulation excipients are eluted. Ultraviolet detection was performed at 210 nm with a retention time for perillyl alcohol of 7 min. The high sensitivity assay utilizes a small (5 μL) injection volume for the accurate and precise analysis of perillyl alcohol from a complex cream formulation.

Original languageEnglish (US)
Pages (from-to)447-452
Number of pages6
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume37
Issue number3
DOIs
StatePublished - Mar 9 2005

Fingerprint

perilla alcohol
Drug Compounding
2-Propanol
High Pressure Liquid Chromatography
Assays
Pharmaceutical Preparations
Excipients
Direct injection
Dilution
Injections
Purification
Lipids
Water
Liquids

Keywords

  • Cream formulation
  • Perillyl alcohol
  • RP-HPLC method
  • Topical formulation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

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title = "An HPLC method for quantitation of perillyl alcohol in a topical pharmaceutical cream formulation",
abstract = "A reverse phase high performance liquid chromatographic method for quantitation of perillyl alcohol in a topical cream pharmaceutical formulation was developed. Previously reported methods for analyzing drugs in lipid formulations are relatively complex and time consuming, with extraction, purification and derivatization involved. Through a simple dilution of the cream formulation in isopropyl alcohol, the present assay method enables the direct injection of the samples, on an Alltima C18 5 μ, 150 mm × 2.1 mm, narrow bore column (Alltech Associates, Deerfield, IL). The method includes an isocratic run with acetonitrile-water (40:60, v/v) at 0.35 mL/min for 12 min, followed by a gradient wash with isopropyl alcohol for 20 min, to ensure that all formulation excipients are eluted. Ultraviolet detection was performed at 210 nm with a retention time for perillyl alcohol of 7 min. The high sensitivity assay utilizes a small (5 μL) injection volume for the accurate and precise analysis of perillyl alcohol from a complex cream formulation.",
keywords = "Cream formulation, Perillyl alcohol, RP-HPLC method, Topical formulation",
author = "Abhishek Gupta and Stratton, {Steven P} and Myrdal, {Paul B}",
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AU - Gupta, Abhishek

AU - Stratton, Steven P

AU - Myrdal, Paul B

PY - 2005/3/9

Y1 - 2005/3/9

N2 - A reverse phase high performance liquid chromatographic method for quantitation of perillyl alcohol in a topical cream pharmaceutical formulation was developed. Previously reported methods for analyzing drugs in lipid formulations are relatively complex and time consuming, with extraction, purification and derivatization involved. Through a simple dilution of the cream formulation in isopropyl alcohol, the present assay method enables the direct injection of the samples, on an Alltima C18 5 μ, 150 mm × 2.1 mm, narrow bore column (Alltech Associates, Deerfield, IL). The method includes an isocratic run with acetonitrile-water (40:60, v/v) at 0.35 mL/min for 12 min, followed by a gradient wash with isopropyl alcohol for 20 min, to ensure that all formulation excipients are eluted. Ultraviolet detection was performed at 210 nm with a retention time for perillyl alcohol of 7 min. The high sensitivity assay utilizes a small (5 μL) injection volume for the accurate and precise analysis of perillyl alcohol from a complex cream formulation.

AB - A reverse phase high performance liquid chromatographic method for quantitation of perillyl alcohol in a topical cream pharmaceutical formulation was developed. Previously reported methods for analyzing drugs in lipid formulations are relatively complex and time consuming, with extraction, purification and derivatization involved. Through a simple dilution of the cream formulation in isopropyl alcohol, the present assay method enables the direct injection of the samples, on an Alltima C18 5 μ, 150 mm × 2.1 mm, narrow bore column (Alltech Associates, Deerfield, IL). The method includes an isocratic run with acetonitrile-water (40:60, v/v) at 0.35 mL/min for 12 min, followed by a gradient wash with isopropyl alcohol for 20 min, to ensure that all formulation excipients are eluted. Ultraviolet detection was performed at 210 nm with a retention time for perillyl alcohol of 7 min. The high sensitivity assay utilizes a small (5 μL) injection volume for the accurate and precise analysis of perillyl alcohol from a complex cream formulation.

KW - Cream formulation

KW - Perillyl alcohol

KW - RP-HPLC method

KW - Topical formulation

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