An immunoreactive apoglycoprotein purified from Coccidioides immitis

K. O. Dugger, J. N. Galgiani, Neil M. Ampel, S. H. Sun, D. M. Magee, J. Harrison, J. H. Law

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Deglycosylation of glycoproteins in a lysate of spherules of Coccidioides immitis has permitted purification and partial characterization of a proline-rich pronase-sensitive antigen. Moreover, soluble antigen specifically stimulated lymphocytes from persons with dermal delayed-type hypersensitivity to coccidioidal antigens. When related to reference coccidioidin by tandem two-dimensional immunoelectrophoresis, the antigen fused in the anodal region with a specific reference antigen (antigen 2). It did not show identity with coccidioidal antigens used in conventional serologic assays. Although immunoblots of the purified protein with monospecific rabbit antiserum showed a single antigen at 33 kDa, the parent spherule lysate bound the same antibody in a broad band between 70 and >200 kDa, which could be explained by microheterogeneity of glycosylation. Immunoelectron microscopy using affinity-purified human antibodies localized the antigen to the cell wall and internal septa of spherules. These findings suggest that the apoglycoprotein may be important in human immune responses to coccidioidal infection.

Original languageEnglish (US)
Pages (from-to)2245-2251
Number of pages7
JournalInfection and Immunity
Volume59
Issue number7
StatePublished - 1991

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Coccidioides
Antigens
Coccidioidin
Two-Dimensional Immunoelectrophoresis
Pronase
Immunoelectron Microscopy
Antibodies
Delayed Hypersensitivity
Glycosylation
Proline
Cell Wall
Immune Sera
Glycoproteins
Lymphocytes
Rabbits

ASJC Scopus subject areas

  • Immunology

Cite this

Dugger, K. O., Galgiani, J. N., Ampel, N. M., Sun, S. H., Magee, D. M., Harrison, J., & Law, J. H. (1991). An immunoreactive apoglycoprotein purified from Coccidioides immitis. Infection and Immunity, 59(7), 2245-2251.

An immunoreactive apoglycoprotein purified from Coccidioides immitis. / Dugger, K. O.; Galgiani, J. N.; Ampel, Neil M.; Sun, S. H.; Magee, D. M.; Harrison, J.; Law, J. H.

In: Infection and Immunity, Vol. 59, No. 7, 1991, p. 2245-2251.

Research output: Contribution to journalArticle

Dugger, KO, Galgiani, JN, Ampel, NM, Sun, SH, Magee, DM, Harrison, J & Law, JH 1991, 'An immunoreactive apoglycoprotein purified from Coccidioides immitis', Infection and Immunity, vol. 59, no. 7, pp. 2245-2251.
Dugger, K. O. ; Galgiani, J. N. ; Ampel, Neil M. ; Sun, S. H. ; Magee, D. M. ; Harrison, J. ; Law, J. H. / An immunoreactive apoglycoprotein purified from Coccidioides immitis. In: Infection and Immunity. 1991 ; Vol. 59, No. 7. pp. 2245-2251.
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