An improved method for plant BAC library construction.

Meizhong Luo, Rod A. Wing

Research output: Contribution to journalArticlepeer-review

83 Scopus citations

Abstract

Large genomic DNA insert-containing libraries are required as critical tools for physical mapping, positional cloning, and genome sequencing of complex genomes. The bacterial artificial chromosome (BAC) cloning system has become a dominant system over others to clone large genomic DNA inserts. As the costs of positional cloning, physical mapping, and genome sequencing continuously decrease, there is an increasing demand for high-quality deep-coverage large insert BAC libraries. In our laboratory, we have constructed many high-quality deep-coverage large insert BAC libraries including arabidopsis, manocot and dicot crop plants, and plant pathogens. Here, we present the protocol used in our laboratory to construct BAC libraries.

Original languageEnglish (US)
Pages (from-to)3-20
Number of pages18
JournalMethods in molecular biology (Clifton, N.J.)
Volume236
DOIs
StatePublished - 2003

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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