An improved method for the concentration of rotaviruses from large volumes of water

Gary A. Toranzos, Charles P. Gerba

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A microporous filter method for the concentration and reconcentration of rotaviruses from large volumes of water was developed. This method allows for the processing of large volumes of water with high recoveries of rotaviruses. Averages of 49% and 41% of seeded viruses were recovered from 20 and 400 l of water, respectively. Of the various eluents evaluated, a mixture of 10% tryptose phosphate broth and 3% beef extract at a pH value of 10.0 was found to give the greatest elution efficiency. The use of the positively charged 1MDS filters allowed for the processing of waters without the need for pH adjustments. An overall recovery of 35% was obtained from 400 l when hydroextraction was used as a secondstep concentration procedure.

Original languageEnglish (US)
Pages (from-to)131-140
Number of pages10
JournalJournal of Virological Methods
Volume24
Issue number1-2
DOIs
StatePublished - Jan 1 1989

Keywords

  • Concentration
  • Rotavirus, recovery from water

ASJC Scopus subject areas

  • Virology

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