An improved Taura syndrome virus (TSV) RT-PCR using newly designed primers

Research output: Contribution to journalArticle

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Abstract

Taura syndrome virus (TSV)-specific primers, designated as 7171F/7511R, were designed to improve the sensitivity of RT-PCR detection. This pair of primers was shown to detect TSV isolates representative of four phylogenetic lineages: Belize, Americas, SE Asia, and Venezuela. Its detection limit was determined to be 20 copies of the TSV genome, 100 times more sensitive than the TSV primers 9992/9195 currently being used by many laboratories and recommended for surveillance and diagnostic applications by the Office International des Epizooties. Primers 7171F/7511R were found to be specific to TSV and did not to react to either Infectious hypodermal and hematopoietic necrosis virus (IHHNV), White spot syndrome virus (WSSV), Yellow head virus (YHV), or Infectious myonecrosis virus (IMNV). This new RT-PCR method was shown to detect TSV in chronically infected Penaeus vannamei that had survived up to 236 days after exposure to TSV.

Original languageEnglish (US)
Pages (from-to)290-292
Number of pages3
JournalAquaculture
Volume293
Issue number3-4
DOIs
StatePublished - Aug 16 2009

Fingerprint

Taura syndrome virus
virus
reverse transcriptase polymerase chain reaction
Gill-associated virus
Infectious hematopoietic necrosis virus
White spot syndrome virus
Belize
Litopenaeus vannamei
Venezuela
detection limit
viruses
genome
monitoring
phylogeny

Keywords

  • Penaeus vannamei
  • Reverse transcriptase-polymerase chain reaction
  • RT-PCR
  • Taura syndrome virus
  • TSV

ASJC Scopus subject areas

  • Aquatic Science

Cite this

An improved Taura syndrome virus (TSV) RT-PCR using newly designed primers. / Navarro, Solangel A.; Tang-Nelson, Feng-Jyu; Lightner, Donald V.

In: Aquaculture, Vol. 293, No. 3-4, 16.08.2009, p. 290-292.

Research output: Contribution to journalArticle

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