An increase in cardiac alpha1-adrenoceptors following chronic clonidine treatment

Shizuo Yamada, Henry I. Yamamura, William R. Roeske

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Chronic treatment (22 days) of rats with clonidine (0.5 mg/kg s.c. twice a day followed by 20 h of withdrawal) resulted in a significant increase in the specific [3H]WB4101 binding to ventricular and intraventricular septal α1-adrenoceptors but no alteration of the atrial α1-adrenoceptors. Scatchard analysis indicated that the increase in the [3H]WB4101 binding to the clonidine-treated cardiac tissues was due to an enhancement of the α1-adrenoceptor density since there was a significant increase in the Bmax value for the [3H]WB4101 binding to the treated ventricles without a change in the Kd value. The specific [3H]WB4101 binding to cardiac α1-adrenoceptors was not altered by the acute (1 day) or 7 days treatment with clonidine. Chronic treatment with clonidine had no significant effect on the specific [3H](-)DHA binding to the atrial and ventricular β-adrenoceptors. The noradrenaline (NA) concentrations in the clonidine-treated ventricles and intraventricular septae were decreased by 16-20%. These data provide biochemical evidence compatible with a significant reduction of sympathetic outflow to the ventricular myocardium by clonidine.

Original languageEnglish (US)
Pages (from-to)115-118
Number of pages4
JournalNaunyn-Schmiedeberg's Archives of Pharmacology
Volume320
Issue number2
DOIs
Publication statusPublished - Aug 1982
Externally publishedYes

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Keywords

  • [H]WB4101 binding
  • Cardiac α-adrenoceptor
  • Chronic clonidine treatment
  • Noradrenaline concentrations
  • Withdrawal syndromes

ASJC Scopus subject areas

  • Pharmacology

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