An informatic framework for decoding protein complexes by top-down mass spectrometry

Owen S. Skinner, Pierre C. Havugimana, Nicole A. Haverland, Luca Fornelli, Bryan P. Early, Joseph B. Greer, Ryan T. Fellers, Kenneth R. Durbin, Luis H F Do Vale, Rafael D. Melani, Henrique S. Seckler, Micah T. Nelp, Mikhail E. Belov, Stevan R. Horning, Alexander A. Makarov, Richard D. LeDuc, Vahe Bandarian, Philip D. Compton, Neil L. Kelleher

Research output: Research - peer-reviewArticle

  • 13 Citations

Abstract

Efforts to map the human protein interactome have resulted in information about thousands of multi-protein assemblies housed in public repositories, but the molecular characterization and stoichiometry of their protein subunits remains largely unknown. Here, we report a computational search strategy that supports hierarchical top-down analysis for precise identification and scoring of multi-proteoform complexes by native mass spectrometry.

LanguageEnglish (US)
Pages237-240
Number of pages4
JournalNature Methods
Volume13
Issue number3
DOIs
StatePublished - Feb 25 2016

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Informatics
Mass Spectrometry
Proteins
Mass spectrometry
Protein Subunits
Stoichiometry

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Biochemistry
  • Cell Biology

Cite this

Skinner, O. S., Havugimana, P. C., Haverland, N. A., Fornelli, L., Early, B. P., Greer, J. B., ... Kelleher, N. L. (2016). An informatic framework for decoding protein complexes by top-down mass spectrometry. Nature Methods, 13(3), 237-240. DOI: 10.1038/nmeth.3731

An informatic framework for decoding protein complexes by top-down mass spectrometry. / Skinner, Owen S.; Havugimana, Pierre C.; Haverland, Nicole A.; Fornelli, Luca; Early, Bryan P.; Greer, Joseph B.; Fellers, Ryan T.; Durbin, Kenneth R.; Do Vale, Luis H F; Melani, Rafael D.; Seckler, Henrique S.; Nelp, Micah T.; Belov, Mikhail E.; Horning, Stevan R.; Makarov, Alexander A.; LeDuc, Richard D.; Bandarian, Vahe; Compton, Philip D.; Kelleher, Neil L.

In: Nature Methods, Vol. 13, No. 3, 25.02.2016, p. 237-240.

Research output: Research - peer-reviewArticle

Skinner, OS, Havugimana, PC, Haverland, NA, Fornelli, L, Early, BP, Greer, JB, Fellers, RT, Durbin, KR, Do Vale, LHF, Melani, RD, Seckler, HS, Nelp, MT, Belov, ME, Horning, SR, Makarov, AA, LeDuc, RD, Bandarian, V, Compton, PD & Kelleher, NL 2016, 'An informatic framework for decoding protein complexes by top-down mass spectrometry' Nature Methods, vol 13, no. 3, pp. 237-240. DOI: 10.1038/nmeth.3731
Skinner OS, Havugimana PC, Haverland NA, Fornelli L, Early BP, Greer JB et al. An informatic framework for decoding protein complexes by top-down mass spectrometry. Nature Methods. 2016 Feb 25;13(3):237-240. Available from, DOI: 10.1038/nmeth.3731
Skinner, Owen S. ; Havugimana, Pierre C. ; Haverland, Nicole A. ; Fornelli, Luca ; Early, Bryan P. ; Greer, Joseph B. ; Fellers, Ryan T. ; Durbin, Kenneth R. ; Do Vale, Luis H F ; Melani, Rafael D. ; Seckler, Henrique S. ; Nelp, Micah T. ; Belov, Mikhail E. ; Horning, Stevan R. ; Makarov, Alexander A. ; LeDuc, Richard D. ; Bandarian, Vahe ; Compton, Philip D. ; Kelleher, Neil L./ An informatic framework for decoding protein complexes by top-down mass spectrometry. In: Nature Methods. 2016 ; Vol. 13, No. 3. pp. 237-240
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