An integrated technique for volume estimation of spots IN 3-D human cell cultures: Watersnakes

Narasimhan Rajagopalan, Jeffrey J. Rodríguez, Kathleen Dixon

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this paper we propose a new integrated technique to accurately measure the volume of 3-D spots in images of human cell cultures by combining watershed transformation and active contour models. We use the watershed contour as the initial snake contour and develop a novel scheme to estimate the snake parameters. Performance of this new technique is measured on synthetic images and images of micro-beads and compared with traditional techniques.

Original languageEnglish (US)
Title of host publication2008 IEEE International Conference on Image Processing, ICIP 2008 Proceedings
Pages3004-3007
Number of pages4
DOIs
StatePublished - Dec 1 2008
Event2008 IEEE International Conference on Image Processing, ICIP 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 15 2008

Publication series

NameProceedings - International Conference on Image Processing, ICIP
ISSN (Print)1522-4880

Other

Other2008 IEEE International Conference on Image Processing, ICIP 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/15/08

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Keywords

  • 3-D active contours
  • Foci detection
  • Snakes
  • Watershed transformation

ASJC Scopus subject areas

  • Software
  • Computer Vision and Pattern Recognition
  • Signal Processing

Cite this

Rajagopalan, N., Rodríguez, J. J., & Dixon, K. (2008). An integrated technique for volume estimation of spots IN 3-D human cell cultures: Watersnakes. In 2008 IEEE International Conference on Image Processing, ICIP 2008 Proceedings (pp. 3004-3007). [4712427] (Proceedings - International Conference on Image Processing, ICIP). https://doi.org/10.1109/ICIP.2008.4712427