An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly

Tiffany A. McLamarrah, Daniel W. Buster, Brian J. Galletta, Cody J. Boese, John M. Ryniawec, Natalie Ann Hollingsworth, Amy E. Byrnes, Christopher W. Brownlee, Kevin C. Slep, Nasser M. Rusan, Gregory C. Rogers

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Polo-like kinase 4 (Plk4) initiates an early step in centriole assembly by phosphorylating Ana2/STIL, a structural component of the procentriole. Here, we show that Plk4 binding to the central coiled-coil (CC) of Ana2 is a conserved event involving Polo-box 3 and a previously unidentified putative CC located adjacent to the kinase domain. Ana2 is then phosphorylated along its length. Previous studies showed that Plk4 phosphorylates the C-terminal STil/ANa2 (STAN) domain of Ana2/ STIL, triggering binding and recruitment of the cartwheel protein Sas6 to the procentriole assembly site. However, the physiological relevance of N-terminal phosphorylation was unknown. We found that Plk4 first phosphorylates the extreme N terminus of Ana2, which is critical for subsequent STAN domain modification. Phosphorylation of the central region then breaks the Plk4-Ana2 interaction. This phosphorylation pattern is important for centriole assembly and integrity because replacement of endogenous Ana2 with phospho-Ana2 mutants disrupts distinct steps in Ana2 function and inhibits centriole duplication.

Original languageEnglish (US)
Pages (from-to)1217-1231
Number of pages15
JournalJournal of Cell Biology
Volume217
Issue number4
DOIs
StatePublished - Apr 1 2018

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Centrioles
Phosphotransferases
Phosphorylation
Proteins

ASJC Scopus subject areas

  • Cell Biology

Cite this

McLamarrah, T. A., Buster, D. W., Galletta, B. J., Boese, C. J., Ryniawec, J. M., Hollingsworth, N. A., ... Rogers, G. C. (2018). An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly. Journal of Cell Biology, 217(4), 1217-1231. https://doi.org/10.1083/jcb.201605106

An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly. / McLamarrah, Tiffany A.; Buster, Daniel W.; Galletta, Brian J.; Boese, Cody J.; Ryniawec, John M.; Hollingsworth, Natalie Ann; Byrnes, Amy E.; Brownlee, Christopher W.; Slep, Kevin C.; Rusan, Nasser M.; Rogers, Gregory C.

In: Journal of Cell Biology, Vol. 217, No. 4, 01.04.2018, p. 1217-1231.

Research output: Contribution to journalArticle

McLamarrah, TA, Buster, DW, Galletta, BJ, Boese, CJ, Ryniawec, JM, Hollingsworth, NA, Byrnes, AE, Brownlee, CW, Slep, KC, Rusan, NM & Rogers, GC 2018, 'An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly', Journal of Cell Biology, vol. 217, no. 4, pp. 1217-1231. https://doi.org/10.1083/jcb.201605106
McLamarrah TA, Buster DW, Galletta BJ, Boese CJ, Ryniawec JM, Hollingsworth NA et al. An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly. Journal of Cell Biology. 2018 Apr 1;217(4):1217-1231. https://doi.org/10.1083/jcb.201605106
McLamarrah, Tiffany A. ; Buster, Daniel W. ; Galletta, Brian J. ; Boese, Cody J. ; Ryniawec, John M. ; Hollingsworth, Natalie Ann ; Byrnes, Amy E. ; Brownlee, Christopher W. ; Slep, Kevin C. ; Rusan, Nasser M. ; Rogers, Gregory C. / An ordered pattern of Ana2 phosphorylation by Plk4 is required for centriole assembly. In: Journal of Cell Biology. 2018 ; Vol. 217, No. 4. pp. 1217-1231.
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AU - Rusan, Nasser M.

AU - Rogers, Gregory C.

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