Analyses of the spleen proteome of chickens infected with Marek's disease virus

Niroshan Thanthrige-Don, Mohamed F. Abdul-Careem, L. Allen Shack, Shane C Burgess, Shayan Sharif

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Marek's disease virus (MDV), which causes a lymphoproliferative disease in chickens, is known to induce host responses leading to protection against disease in a manner dependent on genetic background of chickens and virulence of the virus. In the present study, changes in the spleen proteome at 7, 14 and 21 days post-infection in response to MDV infection were studied using two-dimensional polyacrylamide gel electrophoresis. Differentially expressed proteins were identified using one-dimensional liquid chromatography electrospray ionization tandem mass spectrometry (1D LC ESI MS/MS). Comparative analysis of multiple gels revealed that the majority of changes had occurred at early stages of the disease. In total, 61 protein spots representing 48 host proteins were detected as either quantitatively (false discovery rate (FDR) ≤ 0.05 and fold change ≥ 2) or qualitatively differentially expressed at least once during different sampling points. Overall, the proteins identified in the present study are involved in a variety of cellular processes such as the antigen processing and presentation, ubiquitin-proteasome protein degradation (UPP), formation of the cytoskeleton, cellular metabolism, signal transduction and regulation of translation. Notably, early stages of the disease were characterized by changes in the UPP, and antigen presentation. Furthermore, changes indicative of active cell proliferation as well as apoptosis together with significant changes in cytoskeletal components that were observed throughout the experimental period suggested the complexity of the pathogenesis. The present findings provide a basis for further studies aimed at elucidation of the role of these proteins in MDV interactions with its host.

Original languageEnglish (US)
Pages (from-to)356-367
Number of pages12
JournalVirology
Volume390
Issue number2
DOIs
StatePublished - Aug 1 2009
Externally publishedYes

Fingerprint

Marek Disease
Proteome
Chickens
Spleen
Viruses
Antigen Presentation
Proteasome Endopeptidase Complex
Ubiquitin
Proteins
Proteolysis
Electrospray Ionization Mass Spectrometry
Electrophoresis, Gel, Two-Dimensional
Virus Diseases
Tandem Mass Spectrometry
Cytoskeleton
Liquid Chromatography
Virulence
Signal Transduction
Gels
Cell Proliferation

Keywords

  • 1D LC ESI MS/MS
  • 2D gel electrophoresis
  • Chicken proteome
  • Host response
  • Marek's disease virus

ASJC Scopus subject areas

  • Virology

Cite this

Analyses of the spleen proteome of chickens infected with Marek's disease virus. / Thanthrige-Don, Niroshan; Abdul-Careem, Mohamed F.; Shack, L. Allen; Burgess, Shane C; Sharif, Shayan.

In: Virology, Vol. 390, No. 2, 01.08.2009, p. 356-367.

Research output: Contribution to journalArticle

Thanthrige-Don, N, Abdul-Careem, MF, Shack, LA, Burgess, SC & Sharif, S 2009, 'Analyses of the spleen proteome of chickens infected with Marek's disease virus', Virology, vol. 390, no. 2, pp. 356-367. https://doi.org/10.1016/j.virol.2009.05.020
Thanthrige-Don, Niroshan ; Abdul-Careem, Mohamed F. ; Shack, L. Allen ; Burgess, Shane C ; Sharif, Shayan. / Analyses of the spleen proteome of chickens infected with Marek's disease virus. In: Virology. 2009 ; Vol. 390, No. 2. pp. 356-367.
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