Analysis and stability of retinol in plasma

Y. M. Peng, M. J. Xu, David S Alberts

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

A simple, precise, and specific high-performance liquid chromatography (HPLC) method was developed for the simultaneous measurement of retinol (ROH), 13-cis-retinoic acid (13-cRA), and 4-oxo-13-cRA. The average recovery of ROH from serum or plasma was 95%, and the precision of the assay was less than 5%. With this HPLC method, a series of studies was carried out to evaluate the stability of ROH in various matrices, ROH was stable under our HPLC assay conditions as well as in plasma- and in serum-enriched culture media; however, ROH was not stable in aqueous matrices. Serum or heparinized plasma may be routinely used for measurement of ROH concentrations, providing EDTA, oxalate, and citrate are not used as anticoagulants. Because of ROH stability, blood samples can be kept on ice in the dark for at least 24 hours prior to separation of plasma. In addition, plasma samples containing ROH can be stored for up to 1 year at -20°C without loss of stability.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalJournal of the National Cancer Institute
Volume78
Issue number1
StatePublished - 1987

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Vitamin A
High Pressure Liquid Chromatography
Serum
Isotretinoin
Oxalates
Ice
Edetic Acid
Citric Acid
Anticoagulants
Culture Media

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Analysis and stability of retinol in plasma. / Peng, Y. M.; Xu, M. J.; Alberts, David S.

In: Journal of the National Cancer Institute, Vol. 78, No. 1, 1987, p. 95-99.

Research output: Contribution to journalArticle

Peng, Y. M. ; Xu, M. J. ; Alberts, David S. / Analysis and stability of retinol in plasma. In: Journal of the National Cancer Institute. 1987 ; Vol. 78, No. 1. pp. 95-99.
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