Analysis of 4-methylpyrazole in plasma and urine by gas chromatography with nitrogen-selective detection

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A simple, sensitive, and specific gas chromatographic method for the quantitation of 4-methylpyrazole in plasma and urine is described. Samples containing 4-methylpyrazole, with 3-methylpyrazole as the internal standard, are extracted into ether and the concentrated ethereal extracts are chromatographed on a Carbowax 20M column using nitrogen-selective detection. Standard curves are linear and reproducible over the range of 25-1000 ng/mL for plasma and 0.5-5 μg/mL for urine. Recovery of 4-methylpyrazole is complete from plasma and urine, and the overall between-day coefficient of variation is within 6.0%. No interference is observed from the extractive constituents of plasma and urine. The assay method is suitable for an examination of 4-methylpyrazole disposition in animals and humans.

Original languageEnglish (US)
Pages (from-to)690-692
Number of pages3
JournalJournal of Pharmaceutical Sciences
Volume73
Issue number5
StatePublished - 1984

Fingerprint

Plasma Gases
Gas chromatography
Gas Chromatography
Nitrogen
Urine
Plasmas
Ether
Assays
Animals
Gases
Recovery
fomepizole

ASJC Scopus subject areas

  • Molecular Medicine
  • Chemistry(all)
  • Organic Chemistry
  • Drug Discovery
  • Pharmaceutical Science
  • Pharmacology

Cite this

Analysis of 4-methylpyrazole in plasma and urine by gas chromatography with nitrogen-selective detection. / Achari, R.; Mayersohn, Michael.

In: Journal of Pharmaceutical Sciences, Vol. 73, No. 5, 1984, p. 690-692.

Research output: Contribution to journalArticle

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