A simple, sensitive, and specific gas chromatographic method for the quantitation of 4‐methylpyrazole in plasma and urine is described. Samples containing 4‐methylpyrazole, with 3‐methylpyrazole as the internal standard, are extracted into ether and the concentrated ethereal extracts are chromatographed on a Carbowax 20M column using nitrogen‐selective detection. Standard curves are linear and reproducible over the range of 25–1000 ng/mL for plasma and 0.5–5 μg/mL for urine. Recovery of 4‐methylpyrazole is complete from plasma and urine, and the overall between‐day coefficient of variation is within 6.0%. No interference is observed from the extractive constituents of plasma and urine. The assay method is suitable for an examination of 4‐methylpyrazole disposition in animals and humans.
- 4‐Methylpyrazole—analysis in urine and plasma, gas chromatography with nitrogen‐selective detection
- Gas chromatography—analysis of 4‐methylpyrazole in plasma and urine, nitrogen‐selective detection
ASJC Scopus subject areas
- Pharmaceutical Science