Analysis of bacterial contamination in different sections of a high-purity water system

M. B. McAlister, L. A. Kulakov, M. J. Larkin, Kimberly L Ogden

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Microbial contamination of high-purity water is a significant problem in semiconductor, pharmaceutical and food and beverage industries. Bacterial contamination in different parts of a high-purity water system was studied using plate counts, epifluorescence microscopy, and 16SrRNA gene sequencing. Oligotrophic bacteria strains were classified. Methods requiring direct culture of the bacteria failed to correctly estimate the extent of bacterial contamination. Use of membrane filtration followed by epifluorescence microscopy allowed the rapid and accurate determination of biocontamination levels.

Original languageEnglish (US)
Pages (from-to)18-26
Number of pages9
JournalUltrapure Water
Volume18
Issue number1
StatePublished - 2001

Fingerprint

Contamination
Water
microscopy
Bacteria
Microscopic examination
bacterium
Beverages
food industry
water
Drug products
drug
Genes
Semiconductor materials
membrane
Membranes
gene
Pharmaceutical Preparations
analysis
contamination
Industry

Keywords

  • Bacterium
  • Pollution
  • Water supply

ASJC Scopus subject areas

  • Fluid Flow and Transfer Processes
  • Water Science and Technology

Cite this

Analysis of bacterial contamination in different sections of a high-purity water system. / McAlister, M. B.; Kulakov, L. A.; Larkin, M. J.; Ogden, Kimberly L.

In: Ultrapure Water, Vol. 18, No. 1, 2001, p. 18-26.

Research output: Contribution to journalArticle

McAlister, MB, Kulakov, LA, Larkin, MJ & Ogden, KL 2001, 'Analysis of bacterial contamination in different sections of a high-purity water system', Ultrapure Water, vol. 18, no. 1, pp. 18-26.
McAlister, M. B. ; Kulakov, L. A. ; Larkin, M. J. ; Ogden, Kimberly L. / Analysis of bacterial contamination in different sections of a high-purity water system. In: Ultrapure Water. 2001 ; Vol. 18, No. 1. pp. 18-26.
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