Analysis of gene expression profiles in response to Sclerotinia sclerotiorum in Brassica napus

Jianwei Zhao, Jianlin Wang, Lingling An, R. W. Doerge, Z. Jeffrey Chen, Craig R. Grau, Jinling Meng, Thomas C. Osborn

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Sclerotinia sclerotiorum is a necrotrophic plant pathogen which causes serious disease in agronomically important crop species. The molecular basis of plant defense to this pathogen is poorly understood. We investigated gene expression changes associated with S. sclerotiorum infection in a partially resistant and a susceptible genotype of oilseed Brassica napus using a whole genome microarray from Arabidopsis. A total of 686 and 1,547 genes were found to be differentially expressed after infection in the resistant and susceptible genotypes, respectively. The number of differentially expressed genes increased over infection time with the majority being up-regulated in both genotypes. The putative functions of the differentially expressed genes included pathogenesis-related (PR) proteins, proteins involved in the oxidative burst, protein kinase, molecule transporters, cell maintenance and development, abiotic stress, as well as proteins with unknown functions. The gene regulation patterns indicated that a large part of the defense response exhibited as a temporal and quantitative difference between the two genotypes. Genes associated with jasmonic acid (JA) and ethylene signal transduction pathways were induced, but no salicylic acid (SA) responsive genes were identified. Candidate defense genes were identified by integration of the early response genes in the partially resistant line with previously mapped quantitative trait loci (QTL). Expression levels of these genes were verified by Northern blot analyses. These results indicate that genes encoding various proteins involved in diverse roles, particularly WRKY transcription factors and plant cell wall related proteins may play an important role in the defense response to S. sclerotiorum disease.

Original languageEnglish (US)
Pages (from-to)13-24
Number of pages12
JournalPlanta
Volume227
Issue number1
DOIs
StatePublished - Dec 2007
Externally publishedYes

Fingerprint

Brassica napus
Ascomycota
Sclerotinia sclerotiorum
Transcriptome
gene expression
Genes
genes
Genotype
genotype
Proteins
Infection
proteins
Gene Expression
infection
Respiratory Burst
Salicylic Acid
Quantitative Trait Loci
Plant Cells
pathogenesis-related proteins
Arabidopsis

Keywords

  • Arabidopsis
  • Brassica
  • Gene expression profile
  • Microarray
  • Sclerotinia

ASJC Scopus subject areas

  • Plant Science

Cite this

Zhao, J., Wang, J., An, L., Doerge, R. W., Chen, Z. J., Grau, C. R., ... Osborn, T. C. (2007). Analysis of gene expression profiles in response to Sclerotinia sclerotiorum in Brassica napus. Planta, 227(1), 13-24. https://doi.org/10.1007/s00425-007-0586-z

Analysis of gene expression profiles in response to Sclerotinia sclerotiorum in Brassica napus. / Zhao, Jianwei; Wang, Jianlin; An, Lingling; Doerge, R. W.; Chen, Z. Jeffrey; Grau, Craig R.; Meng, Jinling; Osborn, Thomas C.

In: Planta, Vol. 227, No. 1, 12.2007, p. 13-24.

Research output: Contribution to journalArticle

Zhao, J, Wang, J, An, L, Doerge, RW, Chen, ZJ, Grau, CR, Meng, J & Osborn, TC 2007, 'Analysis of gene expression profiles in response to Sclerotinia sclerotiorum in Brassica napus', Planta, vol. 227, no. 1, pp. 13-24. https://doi.org/10.1007/s00425-007-0586-z
Zhao, Jianwei ; Wang, Jianlin ; An, Lingling ; Doerge, R. W. ; Chen, Z. Jeffrey ; Grau, Craig R. ; Meng, Jinling ; Osborn, Thomas C. / Analysis of gene expression profiles in response to Sclerotinia sclerotiorum in Brassica napus. In: Planta. 2007 ; Vol. 227, No. 1. pp. 13-24.
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