Analysis of heavy metal deposition in renal tissue by sectional mapping using PIXE

R. L. Keith, A Jay Gandolfi, L. C. McIntyre, M. D. Ashbaugh, Q. Fernando

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

This study uses particle induced X-ray emission spectroscopy (PIXE) to examine the localization of metals in renal tissue removed from rabbits exposed to HgCl2, K2Cr2O7, or NaAsO2, or a binary combination. A small beam diameter (10 μm) and the ability to raster this beam over areas of up to 125 μm×125 μm has allowed the mapping of non-endogenous metal concentrations over the entire cross-section of the kidney. The use of step-wise sectional mapping has produced data and revealed trends in heavy metal deposition that are difficult to reproduce with other analytical methods. The combination of small beam size, high resolution, and simultaneous multi-element detection makes PIXE spectroscopy a powerful tool for investigating the impact of non-endogenous metals on the kidney.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalNuclear Instruments and Methods in Physics Research, Section B: Beam Interactions with Materials and Atoms
Volume149
Issue number1-2
Publication statusPublished - Jan 3 1999

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ASJC Scopus subject areas

  • Surfaces, Coatings and Films
  • Instrumentation
  • Surfaces and Interfaces

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