Analysis of Host Responses to Neisseria gonorrhoeae Using a Human Three-Dimensional Endometrial Epithelial Cell Model

Paweł Łaniewski, Melissa M. Herbst-Kralovetz

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Neisseria gonorrhoeae infections have been associated with complications including chronic endometritis and pelvic inflammatory disease. Robust in vitro models of the female reproductive tract are urgently needed to better understand the biological mechanisms leading to these pathophysiological changes. Our human three-dimensional (3D) endometrial epithelial cell (EEC) model, which is generated using the HEC-1A cell line and rotating wall vessel (RWV) bioreactor technology, replicates several hallmarks of endometrial tissue in vivo. Studying the interactions of N. gonorrhoeae with the host using this newly characterized human 3D EEC model allows for the investigation of unique mechanisms of gonococcal pathogenesis in the upper female reproductive tract. In this chapter, we describe methodologies that can be used to investigate the interactions of N. gonorrhoeae with the human 3D endometrial epithelium. Protocols for generating the human 3D EEC model using the RWV technology and assessing the host response (including morphological/ultrastructural changes to the epithelial cells; cytokine/chemokine secretion or gene expression changes) following infection with N. gonorrhoeae are presented.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages347-361
Number of pages15
DOIs
StatePublished - Jan 1 2019

Publication series

NameMethods in Molecular Biology
Volume1997
ISSN (Print)1064-3745

Keywords

  • Female reproductive tract
  • Host–pathogen interactions
  • Human epithelial cells
  • Innate immunity
  • Neisseria gonorrhoeae
  • Organotypic mucosal model
  • Rotating wall vessel bioreactor
  • Uterine infection

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Łaniewski, P., & Herbst-Kralovetz, M. M. (2019). Analysis of Host Responses to Neisseria gonorrhoeae Using a Human Three-Dimensional Endometrial Epithelial Cell Model. In Methods in Molecular Biology (pp. 347-361). (Methods in Molecular Biology; Vol. 1997). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-9496-0_20