Analysis of Membrane-Bound Organelles

Hanna Fares, Alexander M. van der Bliek

Research output: Chapter in Book/Report/Conference proceedingChapter

3 Scopus citations

Abstract

This chapter describes methods for studying membrane traffic and organelle biogenesis in Caenorhabditis elegans. These processes have traditionally been studied with yeast or mammalian cells, but C. elegans is emerging as an attractive alternative model system for cell biologists. C. elegans is well known for the ease of manipulation through classic and molecular genetic techniques. In addition, C. elegans is transparent, so fluorescent proteins can be observed in live animals. These properties have aided the development of functional assays for tracking cell biological processes in situ. Localization results obtained with fluorescent proteins can be validated with immunofluorescence and with biochemical methods, such as subcellular fractionation, adapted from methods developed for other organisms. C. elegans thus combines powerful genetics with a range of cell biological techniques to study subcellular processes in a tractable multicellular organism.

Original languageEnglish (US)
Title of host publicationMethods in Cell Biology
PublisherAcademic Press Inc.
Pages239-263
Number of pages25
DOIs
StatePublished - 2012

Publication series

NameMethods in Cell Biology
Volume107
ISSN (Print)0091-679X

Keywords

  • Construct
  • Endocytosis
  • Epitope
  • Lysosomal
  • Mapping
  • Photobleaching

ASJC Scopus subject areas

  • Cell Biology

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