Analysis of mode of reproduction of guayule (Parthenium argentatum A. Gray) using flow cytometry and identification of polyhaploids for breeding

Von Mark V. Cruz, Amber L. Lynch, Dennis T. Ray, William S. Niaura, Philip H. Purdy, David A. Dierig

Research output: Research - peer-reviewArticle

Abstract

Guayule (Parthenium argentatum A. Gray) is a promising domestic source of solid rubber for tire manufacturing in North America. Crop improvement and breeding efforts on guayule have enabled improved germplasm to be developed and deployed on commercial fields. One difficulty in guayule breeding is the identification of the mode of reproduction, particularly in polyploids, which exhibit a mixed mode due to their facultative nature. Guayule polyploid plants range from being triploids (3n = 3x = 54) to octaploids (8n = 8x = 144). Polyploids are known to reproduce by facultative apomixis, while diploids (2n = 2x = 36) follow a sexual mode of reproduction. Flow cytometry is a useful tool for determining ploidy levels of plants. One objective of this study was to estimate the rate of apomixis occurring in different USDA germplasm lines, as well as whether or not there was a meiotic reduction in the megaspore mother cell, and if there was fertilization to form an embryo. We examined the leaf tissue of maternal plants and 100 progeny seeds from these plants, to estimate their rate of apomixis. Combined ploidy information from two different seed tissues (embryo and endosperm) was utilized. Flow cytometric analysis of polyhaploids was also conducted to enable validation of their mode of reproduction, which have not previously been identified in the literature. Results from this study indicated that off-type progeny (seed) would not have been identified if only leaf samples were used, resulting in either, an over-estimation of the rate of apomixis for the accession, or misclassification of the type of reproduction. All identified polyhaploids were analyzed for genetic diversity, and were compared with existing diploid germplasm.

LanguageEnglish (US)
Pages618-623
Number of pages6
JournalIndustrial Crops and Products
Volume107
DOIs
StatePublished - Nov 15 2017

Fingerprint

Parthenium argentatum
guayule
apomixis
flow cytometry
breeding
polyploidy
germplasm
seeds
ploidy
embryo (plant)
diploidy
leaves
tissues
octaploidy
megasporocytes
tires
fertilization (reproduction)
triploidy
rubber
USDA

Keywords

  • Asteraceae
  • Flow cytometry seed screening
  • Genotyping
  • Plant breeding
  • Polyhaploid

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Analysis of mode of reproduction of guayule (Parthenium argentatum A. Gray) using flow cytometry and identification of polyhaploids for breeding. / Cruz, Von Mark V.; Lynch, Amber L.; Ray, Dennis T.; Niaura, William S.; Purdy, Philip H.; Dierig, David A.

In: Industrial Crops and Products, Vol. 107, 15.11.2017, p. 618-623.

Research output: Research - peer-reviewArticle

Cruz, Von Mark V. ; Lynch, Amber L. ; Ray, Dennis T. ; Niaura, William S. ; Purdy, Philip H. ; Dierig, David A./ Analysis of mode of reproduction of guayule (Parthenium argentatum A. Gray) using flow cytometry and identification of polyhaploids for breeding. In: Industrial Crops and Products. 2017 ; Vol. 107. pp. 618-623
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