Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells

Bernard W. Futscher, Mohammad R. Abbaszadegan, Frederick Domann, William S. Dalton

Research output: Contribution to journalArticle

72 Scopus citations

Abstract

MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.

Original languageEnglish (US)
Pages (from-to)1601-1606
Number of pages6
JournalBiochemical Pharmacology
Volume47
Issue number9
DOIs
StatePublished - Apr 29 1994

Keywords

  • MRP
  • PCR
  • drug resistance
  • human tumor cell lines
  • mRNA
  • mitoxantrone

ASJC Scopus subject areas

  • Biochemistry
  • Pharmacology

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