Abstract
MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 1601-1606 |
| Number of pages | 6 |
| Journal | Biochemical Pharmacology |
| Volume | 47 |
| Issue number | 9 |
| DOIs | |
| State | Published - Apr 29 1994 |
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Keywords
- drug resistance
- human tumor cell lines
- mitoxantrone
- mRNA
- MRP
- PCR
ASJC Scopus subject areas
- Pharmacology
Cite this
Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells. / Futscher, Bernard W; Abbaszadegan, Mohammad R.; Domann, Frederick; Dalton, William S.
In: Biochemical Pharmacology, Vol. 47, No. 9, 29.04.1994, p. 1601-1606.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells
AU - Futscher, Bernard W
AU - Abbaszadegan, Mohammad R.
AU - Domann, Frederick
AU - Dalton, William S.
PY - 1994/4/29
Y1 - 1994/4/29
N2 - MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.
AB - MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.
KW - drug resistance
KW - human tumor cell lines
KW - mitoxantrone
KW - mRNA
KW - MRP
KW - PCR
UR - http://www.scopus.com/inward/record.url?scp=0028239105&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028239105&partnerID=8YFLogxK
U2 - 10.1016/0006-2952(94)90538-X
DO - 10.1016/0006-2952(94)90538-X
M3 - Article
C2 - 8185674
AN - SCOPUS:0028239105
VL - 47
SP - 1601
EP - 1606
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 9
ER -