Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells

Bernard W. Futscher; Mohammad R. Abbaszadegan; Frederick Domann; William S. Dalton

doi:10.1016/0006-2952(94)90538-X

Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells

Bernard W. Futscher, Mohammad R. Abbaszadegan, Frederick Domann, William S. Dalton

Pharmacology and Toxicology

Research output: Contribution to journal › Article

72 Scopus citations

Abstract

MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.

Original language	English (US)
Pages (from-to)	1601-1606
Number of pages	6
Journal	Biochemical Pharmacology
Volume	47
Issue number	9
DOIs	https://doi.org/10.1016/0006-2952(94)90538-X
State	Published - Apr 29 1994

Keywords

MRP
PCR
drug resistance
human tumor cell lines
mRNA
mitoxantrone

ASJC Scopus subject areas

Biochemistry
Pharmacology

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Futscher, B. W., Abbaszadegan, M. R., Domann, F., & Dalton, W. S. (1994). Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells. Biochemical Pharmacology, 47(9), 1601-1606. https://doi.org/10.1016/0006-2952(94)90538-X

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title = "Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells",

abstract = "MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.",

keywords = "MRP, PCR, drug resistance, human tumor cell lines, mRNA, mitoxantrone",

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AU - Dalton, William S.

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N2 - MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.

AB - MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.

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