TY - JOUR
T1 - Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells
AU - Futscher, Bernard W.
AU - Abbaszadegan, Mohammad R.
AU - Domann, Frederick
AU - Dalton, William S.
N1 - Funding Information:
Acknowledgernents--The authors would like to thank Jeffery S. Painter for northern blot analysis of MRP, Mary Gleason-Guzman for tissue culture expertise, and Nils Foley for western blot analysis of PGP. This work was supported in part by NC1 Grants 17094 and 43043 to W.S.D.
PY - 1994/4/29
Y1 - 1994/4/29
N2 - MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.
AB - MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.
KW - MRP
KW - PCR
KW - drug resistance
KW - human tumor cell lines
KW - mRNA
KW - mitoxantrone
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U2 - 10.1016/0006-2952(94)90538-X
DO - 10.1016/0006-2952(94)90538-X
M3 - Article
C2 - 8185674
AN - SCOPUS:0028239105
VL - 47
SP - 1601
EP - 1606
JO - Biochemical Pharmacology
JF - Biochemical Pharmacology
SN - 0006-2952
IS - 9
ER -