Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells

Bernard W Futscher, Mohammad R. Abbaszadegan, Frederick Domann, William S. Dalton

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

MRP, a gene recently isolated from a non-P-glycoprotein-mediated multidrug-resistant small cell lung cancer line, is a candidate multidrug-resistance gene. Mitoxantrone, an anthracenedione antitumor agent, frequently selects for non-P-glycoprotein-mediated multidrug resistance in in vitro models. To determine whether mitoxantrone-selected multidrug resistance wasdue to overexpression of MRP, we examined the expression of MRP in four mitoxantrone-selected, multidrug-resistant human tumor cell lines, using a reverse transcriptase/polymerase chain reaction assay. Results from these experiments suggest that overexpression of MRP does not appear to play a primary role in mitoxantrone-selected multidrug resistance in these cell lines, and that other novel drug-resistance mechanisms are likely.

Original languageEnglish (US)
Pages (from-to)1601-1606
Number of pages6
JournalBiochemical Pharmacology
Volume47
Issue number9
DOIs
StatePublished - Apr 29 1994

Fingerprint

Mitoxantrone
Tumors
Multiple Drug Resistance
Cells
Messenger RNA
Neoplasms
Glycoproteins
Genes
MDR Genes
Anthraquinones
Polymerase chain reaction
RNA-Directed DNA Polymerase
Small Cell Lung Carcinoma
Tumor Cell Line
Reverse Transcriptase Polymerase Chain Reaction
Drug Resistance
Antineoplastic Agents
Assays
Cell Line
Pharmaceutical Preparations

Keywords

  • drug resistance
  • human tumor cell lines
  • mitoxantrone
  • mRNA
  • MRP
  • PCR

ASJC Scopus subject areas

  • Pharmacology

Cite this

Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells. / Futscher, Bernard W; Abbaszadegan, Mohammad R.; Domann, Frederick; Dalton, William S.

In: Biochemical Pharmacology, Vol. 47, No. 9, 29.04.1994, p. 1601-1606.

Research output: Contribution to journalArticle

Futscher, Bernard W ; Abbaszadegan, Mohammad R. ; Domann, Frederick ; Dalton, William S. / Analysis of MRP mRNA in mitoxantrone-selected, multidrug-resistant human tumor cells. In: Biochemical Pharmacology. 1994 ; Vol. 47, No. 9. pp. 1601-1606.
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