Analysis of nuclear DNA content and ploidy in higher plants.

D. W. Galbraith, G. M. Lambert, J. Macas, J. Dolezel

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

This is the first of a series of units discussing the application of cytometry to plant material. Techniques commonly used for mammalian nuclei evaluation need considerable modification to be successful with plant material. David Galbraith and his colleagues bring together many years of knowledge in plant cytometry. Their unit provides detailed protocols on measuring DNA content, ploidy, and cell cycle status of plant tissue using both conventional laser based instruments as well as arc lamp cytometers. This unit provides an excellent starting point for those interested in doing cytometry with plants.

Original languageEnglish (US)
Pages (from-to)Unit 7.6
JournalCurrent protocols in cytometry / editorial board, J. Paul Robinson, managing editor ... [et al.]
VolumeChapter 7
StatePublished - May 2001

ASJC Scopus subject areas

  • Biochemistry
  • Histology
  • Medical Laboratory Technology

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