Analysis of the Antitumor Agent Bay i 7433 (Copovithane) in Plasma and Urine by High Performance Liquid Chromatography

Michael G. Rosenblum, Evan M. Hersh, W. Wingender

Research output: Contribution to journalArticle

2 Scopus citations


Copovithane (BAYi7433) is a synthetic polymer of molecular weight 7,800 daltons with antitumor activity. An analytical method for copovithane in biological fluids involving organic extraction, and hydrolysis and TNBS derivitization of generated methylamine was developed. This method utilizes HPLC for final quantitation of the TNBS-methylamine adduct. The lower limit of detection was 15 g/ml of either plasma or urine. This method was sensitive enough to monitor the pharmacokinetics of copovithane in patients receiving therapy.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalJournal of Liquid Chromatography
Issue number1
StatePublished - Jan 1 1984


ASJC Scopus subject areas

  • Molecular Medicine

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