Analysis of the antitumor agent Bay i 7433 (copovithane) in plasma and urine by high performance liquid chromatography

M. G. Rosenblum, Evan M Hersh, W. Wingender, E. M. Hersh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Copovithane (BAYi7433) is a synthetic polymer of molecular weight 7,800 daltons with antitumor activity. An analytical method for copovithane in biological fluids involving organic extraction, and hydrolysis and TNBS derivitization of generated methylamine was developed. This method utilizes HPLC for final quantitation of the TNBS-methylamine adduct. The lower limit of detection was 15 g/ml of either plasma or urine. This method was sensitive enough to monitor the pharmacokinetics of copovithane in patients receiving therapy.

Original languageEnglish (US)
Pages (from-to)159-166
Number of pages8
JournalJournal of Liquid Chromatography
Volume7
Issue number1
StatePublished - 1984
Externally publishedYes

Fingerprint

High performance liquid chromatography
Antineoplastic Agents
High Pressure Liquid Chromatography
Urine
Plasmas
Pharmacokinetics
Limit of Detection
Hydrolysis
Polymers
Molecular Weight
Molecular weight
Fluids
copovithane
methylamine
Therapeutics

ASJC Scopus subject areas

  • Molecular Medicine

Cite this

Analysis of the antitumor agent Bay i 7433 (copovithane) in plasma and urine by high performance liquid chromatography. / Rosenblum, M. G.; Hersh, Evan M; Wingender, W.; Hersh, E. M.

In: Journal of Liquid Chromatography, Vol. 7, No. 1, 1984, p. 159-166.

Research output: Contribution to journalArticle

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