Analysis of the distribution of the major soybean seed allergens in a core collection of Glycine max accessions

R. W. Yaklich, R. M. Helm, G. Cockrell, Eliot M Herman

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

P34 is an outlying member of the papain-superfamily of cysteine proteases that is expressed in developing soybean [Glycine max (L.) Merr.] seeds and may be involved in the defense against Pseudomonas infection. P34 is the major human allergen of soybean seeds and is present in processed food products that contain soybean protein. We have surveyed a core collection of soybean accessions using a monoclonal antibody against P34 and with human sera from soybean-sensitive individuals. We found that the accessions of soybean surveyed contain similar levels of P34 and that P34 is the major allergenic protein. We have also surveyed wild relatives of soybean and found that P34 was present in the examples assayed. These results indicate that it may not be possible to eliminate P34 from the food supply by breeding with an improved germplasm base.

Original languageEnglish (US)
Pages (from-to)1444-1447
Number of pages4
JournalCrop Science
Volume39
Issue number5
StatePublished - Sep 1999
Externally publishedYes

Fingerprint

allergens
Glycine max
soybeans
seeds
papain
cysteine proteinases
wild relatives
processed foods
soy protein
Pseudomonas
monoclonal antibodies
germplasm
foods
breeding
infection
proteins

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Analysis of the distribution of the major soybean seed allergens in a core collection of Glycine max accessions. / Yaklich, R. W.; Helm, R. M.; Cockrell, G.; Herman, Eliot M.

In: Crop Science, Vol. 39, No. 5, 09.1999, p. 1444-1447.

Research output: Contribution to journalArticle

@article{8af543abfe3b47d5a44725736cc7cc1c,
title = "Analysis of the distribution of the major soybean seed allergens in a core collection of Glycine max accessions",
abstract = "P34 is an outlying member of the papain-superfamily of cysteine proteases that is expressed in developing soybean [Glycine max (L.) Merr.] seeds and may be involved in the defense against Pseudomonas infection. P34 is the major human allergen of soybean seeds and is present in processed food products that contain soybean protein. We have surveyed a core collection of soybean accessions using a monoclonal antibody against P34 and with human sera from soybean-sensitive individuals. We found that the accessions of soybean surveyed contain similar levels of P34 and that P34 is the major allergenic protein. We have also surveyed wild relatives of soybean and found that P34 was present in the examples assayed. These results indicate that it may not be possible to eliminate P34 from the food supply by breeding with an improved germplasm base.",
author = "Yaklich, {R. W.} and Helm, {R. M.} and G. Cockrell and Herman, {Eliot M}",
year = "1999",
month = "9",
language = "English (US)",
volume = "39",
pages = "1444--1447",
journal = "Crop Science",
issn = "0011-183X",
publisher = "Crop Science Society of America",
number = "5",

}

TY - JOUR

T1 - Analysis of the distribution of the major soybean seed allergens in a core collection of Glycine max accessions

AU - Yaklich, R. W.

AU - Helm, R. M.

AU - Cockrell, G.

AU - Herman, Eliot M

PY - 1999/9

Y1 - 1999/9

N2 - P34 is an outlying member of the papain-superfamily of cysteine proteases that is expressed in developing soybean [Glycine max (L.) Merr.] seeds and may be involved in the defense against Pseudomonas infection. P34 is the major human allergen of soybean seeds and is present in processed food products that contain soybean protein. We have surveyed a core collection of soybean accessions using a monoclonal antibody against P34 and with human sera from soybean-sensitive individuals. We found that the accessions of soybean surveyed contain similar levels of P34 and that P34 is the major allergenic protein. We have also surveyed wild relatives of soybean and found that P34 was present in the examples assayed. These results indicate that it may not be possible to eliminate P34 from the food supply by breeding with an improved germplasm base.

AB - P34 is an outlying member of the papain-superfamily of cysteine proteases that is expressed in developing soybean [Glycine max (L.) Merr.] seeds and may be involved in the defense against Pseudomonas infection. P34 is the major human allergen of soybean seeds and is present in processed food products that contain soybean protein. We have surveyed a core collection of soybean accessions using a monoclonal antibody against P34 and with human sera from soybean-sensitive individuals. We found that the accessions of soybean surveyed contain similar levels of P34 and that P34 is the major allergenic protein. We have also surveyed wild relatives of soybean and found that P34 was present in the examples assayed. These results indicate that it may not be possible to eliminate P34 from the food supply by breeding with an improved germplasm base.

UR - http://www.scopus.com/inward/record.url?scp=0032873808&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032873808&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0032873808

VL - 39

SP - 1444

EP - 1447

JO - Crop Science

JF - Crop Science

SN - 0011-183X

IS - 5

ER -