Analysis of the Harvey ras gene in cisplatin-initiated mouse skin tumors by polymerase chain reaction and direct DNA sequencing

Mark A. Nelson, Bernard W. Futscher, Michael R. Loew, G. Tim Bowden

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Cisplatin (cis-dichlorodiammineplatinum (II)) acts as a tumor initiator in the mouse skin model of carcinogenesis. DNA transfection studies suggested that skin tumors initiated by cisplatin contained dominant transforming activity. Since the Harvey-ras (H-ras) gene is known to be activated by point mutations in chemically initiated mouse skin tumors, we used polymerase chain reaction (PCR) and direct DNA sequencing to analyze the DNA sequence of the H-ras gene in twelve different cisplatin-initiated skin tumors. The results of these studies indicated that cisplatin-initiated skin tumors were normal (wild-type) at codons 12, 13, 61 and 117. Thus the transforming activity associated with cisplatin initiated mouse skin tumors was not due to a mutant H-ras gene and this suggests the involvement of other transforming genes during initiation of the mouse skin with cisplatin.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalCancer Letters
Volume65
Issue number1
DOIs
StatePublished - Jul 31 1992

Keywords

  • DNA sequencing
  • DNA transfection
  • Harvey-ras gene
  • cisplatin
  • polymerase chain reaction

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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