Analysis of the Harvey ras gene in cisplatin-initiated mouse skin tumors by polymerase chain reaction and direct DNA sequencing

Mark A Nelson, Bernard W Futscher, Michael R. Loew, G. Tim Bowden

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cisplatin (cis-dichlorodiammineplatinum (II)) acts as a tumor initiator in the mouse skin model of carcinogenesis. DNA transfection studies suggested that skin tumors initiated by cisplatin contained dominant transforming activity. Since the Harvey-ras (H-ras) gene is known to be activated by point mutations in chemically initiated mouse skin tumors, we used polymerase chain reaction (PCR) and direct DNA sequencing to analyze the DNA sequence of the H-ras gene in twelve different cisplatin-initiated skin tumors. The results of these studies indicated that cisplatin-initiated skin tumors were normal (wild-type) at codons 12, 13, 61 and 117. Thus the transforming activity associated with cisplatin initiated mouse skin tumors was not due to a mutant H-ras gene and this suggests the involvement of other transforming genes during initiation of the mouse skin with cisplatin.

Original languageEnglish (US)
Pages (from-to)27-33
Number of pages7
JournalCancer Letters
Volume65
Issue number1
DOIs
StatePublished - Jul 31 1992

Fingerprint

ras Genes
DNA Sequence Analysis
Cisplatin
Polymerase Chain Reaction
Skin
Neoplasms
Oncogenes
Point Mutation
Codon
Carcinogens
Transfection
Carcinogenesis
DNA

Keywords

  • cisplatin
  • DNA sequencing
  • DNA transfection
  • Harvey-ras gene
  • polymerase chain reaction

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Analysis of the Harvey ras gene in cisplatin-initiated mouse skin tumors by polymerase chain reaction and direct DNA sequencing. / Nelson, Mark A; Futscher, Bernard W; Loew, Michael R.; Tim Bowden, G.

In: Cancer Letters, Vol. 65, No. 1, 31.07.1992, p. 27-33.

Research output: Contribution to journalArticle

@article{ef16095d4d8e4229b30f4e5dae088bf2,
title = "Analysis of the Harvey ras gene in cisplatin-initiated mouse skin tumors by polymerase chain reaction and direct DNA sequencing",
abstract = "Cisplatin (cis-dichlorodiammineplatinum (II)) acts as a tumor initiator in the mouse skin model of carcinogenesis. DNA transfection studies suggested that skin tumors initiated by cisplatin contained dominant transforming activity. Since the Harvey-ras (H-ras) gene is known to be activated by point mutations in chemically initiated mouse skin tumors, we used polymerase chain reaction (PCR) and direct DNA sequencing to analyze the DNA sequence of the H-ras gene in twelve different cisplatin-initiated skin tumors. The results of these studies indicated that cisplatin-initiated skin tumors were normal (wild-type) at codons 12, 13, 61 and 117. Thus the transforming activity associated with cisplatin initiated mouse skin tumors was not due to a mutant H-ras gene and this suggests the involvement of other transforming genes during initiation of the mouse skin with cisplatin.",
keywords = "cisplatin, DNA sequencing, DNA transfection, Harvey-ras gene, polymerase chain reaction",
author = "Nelson, {Mark A} and Futscher, {Bernard W} and Loew, {Michael R.} and {Tim Bowden}, G.",
year = "1992",
month = "7",
day = "31",
doi = "10.1016/0304-3835(92)90209-E",
language = "English (US)",
volume = "65",
pages = "27--33",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

TY - JOUR

T1 - Analysis of the Harvey ras gene in cisplatin-initiated mouse skin tumors by polymerase chain reaction and direct DNA sequencing

AU - Nelson, Mark A

AU - Futscher, Bernard W

AU - Loew, Michael R.

AU - Tim Bowden, G.

PY - 1992/7/31

Y1 - 1992/7/31

N2 - Cisplatin (cis-dichlorodiammineplatinum (II)) acts as a tumor initiator in the mouse skin model of carcinogenesis. DNA transfection studies suggested that skin tumors initiated by cisplatin contained dominant transforming activity. Since the Harvey-ras (H-ras) gene is known to be activated by point mutations in chemically initiated mouse skin tumors, we used polymerase chain reaction (PCR) and direct DNA sequencing to analyze the DNA sequence of the H-ras gene in twelve different cisplatin-initiated skin tumors. The results of these studies indicated that cisplatin-initiated skin tumors were normal (wild-type) at codons 12, 13, 61 and 117. Thus the transforming activity associated with cisplatin initiated mouse skin tumors was not due to a mutant H-ras gene and this suggests the involvement of other transforming genes during initiation of the mouse skin with cisplatin.

AB - Cisplatin (cis-dichlorodiammineplatinum (II)) acts as a tumor initiator in the mouse skin model of carcinogenesis. DNA transfection studies suggested that skin tumors initiated by cisplatin contained dominant transforming activity. Since the Harvey-ras (H-ras) gene is known to be activated by point mutations in chemically initiated mouse skin tumors, we used polymerase chain reaction (PCR) and direct DNA sequencing to analyze the DNA sequence of the H-ras gene in twelve different cisplatin-initiated skin tumors. The results of these studies indicated that cisplatin-initiated skin tumors were normal (wild-type) at codons 12, 13, 61 and 117. Thus the transforming activity associated with cisplatin initiated mouse skin tumors was not due to a mutant H-ras gene and this suggests the involvement of other transforming genes during initiation of the mouse skin with cisplatin.

KW - cisplatin

KW - DNA sequencing

KW - DNA transfection

KW - Harvey-ras gene

KW - polymerase chain reaction

UR - http://www.scopus.com/inward/record.url?scp=0026662595&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026662595&partnerID=8YFLogxK

U2 - 10.1016/0304-3835(92)90209-E

DO - 10.1016/0304-3835(92)90209-E

M3 - Article

C2 - 1511406

AN - SCOPUS:0026662595

VL - 65

SP - 27

EP - 33

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 1

ER -