Analysis of the p53 gene in human precancerous actinic keratosis lesions and squamous cell cancers

Mark A. Nelson, Janine G. Einspahr, David S. Alberts, Celia A. Balfour, Julie A. Wymer, Kevin L. Welch, Stuart J. Salasche, Jerry L. Bangert, Thomas M. Grogan, Paul O. Bozzo

Research output: Contribution to journalArticle

146 Scopus citations

Abstract

A biomarker of skin cancer would be beneficial in evaluating the efficacy of potential cancer chemoprevention agents. To this end, we investigated the tumor suppresser gene p53 in precancerous actinic keratosis lesions (AK) and malignant squamous cell carcinomas (SCCs) using polymerase chain reaction and single-strand conformation polymorphism analysis (PCR-SSCP) techniques. In addition, p53 protein expression was evaluated using immunohistochemistical analysis with the PAB 1801 monoclonal antibody. Nine out of 13 (69%) SCCs and 8 of 15 (53%) AKs were positive for p53 mutations. In contrast, normal skin samples were negative for p53 mutations. Sequence analysis of AKs and SCCs showed primarily C to T transition mutations. Nuclear immunochemical staining for p53 was observed in 12 15 (80%) AK and 12 13 (92%) SSCs. These results suggest that p53 mutations may be involved in the malignant conversion of AKs to SCCs and that p53 may be useful as a biomarker to study the potential modulatory effects of cancer chemopreventive agents against skin cancer.

Original languageEnglish (US)
Pages (from-to)23-29
Number of pages7
JournalCancer Letters
Volume85
Issue number1
DOIs
StatePublished - Sep 30 1994

Keywords

  • Biomarker
  • Polymerase chain reaction
  • Precancerous actinic keratosis lesions
  • Squamous cell
  • p53

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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  • Cite this

    Nelson, M. A., Einspahr, J. G., Alberts, D. S., Balfour, C. A., Wymer, J. A., Welch, K. L., Salasche, S. J., Bangert, J. L., Grogan, T. M., & Bozzo, P. O. (1994). Analysis of the p53 gene in human precancerous actinic keratosis lesions and squamous cell cancers. Cancer Letters, 85(1), 23-29. https://doi.org/10.1016/0304-3835(94)90234-8