Analytic performance of two automated nonpretreatment digoxin immunoassays

Barun K. De, Dena D. Booth, Pamela J. Magee, Marcia L. Moore, Teresa M. Preuss, T. Aaron Rose, William L. Roberts

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

The analytic performance of two automated nonpretreatment digoxin methods, AxSYM Digoxin II and Vitros digoxin immunoassays, was assessed. Both assays had analytic sensitivities of less than 0.2 μg/L, were linear from digoxin concentrations of 0.5 to 4.0 μg/L, and showed acceptable precision, with a maximum total coefficient of variation (CV) of 8.9% and 6.4% for the AxSYM and Vitros, respectively. Comparison of the two methods using samples from patients receiving digoxin gave the following relationship: Vitros = 0.91 x AxSYM + 0.23 (r = 0.97, S(y,x) = 0.12). Digoxinlike immunoreactive factor (DLIF) crossreactivity was examined in specimens from patients who had hepatic disease, renal insufficiency, had undergone cardiac surgery, and in neonatal cord blood samples. Minimal crossreactivity was observed for most samples and the average crossreactivity for each group of samples was comparable for the two methods. The recovery of digoxin added to samples from each group of DLIF was similar, except for that from cord blood samples, for which recovery was significantly lower with the AxSYM method. Titration of a digoxin-spiked serum pool with digoxin-immune Fab showed a similar decrease in the measured digoxin concentration for both methods. Overall, the analytic performance characteristics of these two methods were comparable.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalTherapeutic Drug Monitoring
Volume21
Issue number1
DOIs
StatePublished - Feb 1 1999
Externally publishedYes

Keywords

  • Digoxin
  • Digoxin-immune Fab
  • Digoxinlike immunoreactive factor
  • Drug monitoring
  • Immunoassay

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)

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  • Cite this

    De, B. K., Booth, D. D., Magee, P. J., Moore, M. L., Preuss, T. M., Rose, T. A., & Roberts, W. L. (1999). Analytic performance of two automated nonpretreatment digoxin immunoassays. Therapeutic Drug Monitoring, 21(1), 123-128. https://doi.org/10.1097/00007691-199902000-00019