Analytical methods for detection of selected estrogenic compounds in aqueous mixtures

Shane A Snyder, Timothy L. Keith, David A. Verbrugge, Erin M. Snyder, Timothy S. Gross, Kurunthachalam Kannan, John P. Giesy

Research output: Contribution to journalArticle

333 Citations (Scopus)

Abstract

Both natural estrogens and synthetic compounds that mimic estrogen can reach the aquatic environment through wastewater discharges. Because nonylphenol (NP), octylphenol (OP), nonylphenol polyethoxylates (NPE), 17β- estradiol (E2), and ethynylestradiol (EE2) have previously been found to be estrogenic and to occur in wastewater effluents, they were the primary analytes for which the method was developed. Water samples were extracted in situ using solid-phase extraction disks. Analytes were separated by high- pressure liquid chromatography and detected by fluorescence or competitive radioimmunoassay (RIA). Method detection limits (MDLs) using HPLC with fluorescence detection were 11, 2, and 52 ng/L of water for NP, OP, and NPE, respectively. The RIA MDLs for E2 and EE2 were 107 and 53 pg/L, respectively. Samples were collected from four municipal wastewater treatment plants in south central Michigan, eight locations on the Trenton Channel of the Detroit River, MI, and five locations in Lake Mead, NV. Concentrations of NP and OP ranged from less than the MDL to 37 and 0.7 μg/L, respectively. NPE concentrations ranged from less than the MDL to 332 μg/L. Concentrations of E2 and EE2 ranged from less than the MDLs to 3.7 and 0.8 ng/L, respectively.

Original languageEnglish (US)
Pages (from-to)2814-2820
Number of pages7
JournalEnvironmental Science and Technology
Volume33
Issue number16
DOIs
StatePublished - Aug 15 1999
Externally publishedYes

Fingerprint

estrogenic compound
detection method
analytical method
Estrogens
Wastewater
Fluorescence
High pressure liquid chromatography
Wastewater treatment
Lakes
Water
Effluents
Rivers
fluorescence
wastewater
Estradiol Congeners
aquatic environment
liquid chromatography
detection
nonylphenol
Estradiol

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Environmental Engineering

Cite this

Snyder, S. A., Keith, T. L., Verbrugge, D. A., Snyder, E. M., Gross, T. S., Kannan, K., & Giesy, J. P. (1999). Analytical methods for detection of selected estrogenic compounds in aqueous mixtures. Environmental Science and Technology, 33(16), 2814-2820. https://doi.org/10.1021/es981294f

Analytical methods for detection of selected estrogenic compounds in aqueous mixtures. / Snyder, Shane A; Keith, Timothy L.; Verbrugge, David A.; Snyder, Erin M.; Gross, Timothy S.; Kannan, Kurunthachalam; Giesy, John P.

In: Environmental Science and Technology, Vol. 33, No. 16, 15.08.1999, p. 2814-2820.

Research output: Contribution to journalArticle

Snyder, SA, Keith, TL, Verbrugge, DA, Snyder, EM, Gross, TS, Kannan, K & Giesy, JP 1999, 'Analytical methods for detection of selected estrogenic compounds in aqueous mixtures', Environmental Science and Technology, vol. 33, no. 16, pp. 2814-2820. https://doi.org/10.1021/es981294f
Snyder, Shane A ; Keith, Timothy L. ; Verbrugge, David A. ; Snyder, Erin M. ; Gross, Timothy S. ; Kannan, Kurunthachalam ; Giesy, John P. / Analytical methods for detection of selected estrogenic compounds in aqueous mixtures. In: Environmental Science and Technology. 1999 ; Vol. 33, No. 16. pp. 2814-2820.
@article{6f1a0973517c4240a07370503ed61806,
title = "Analytical methods for detection of selected estrogenic compounds in aqueous mixtures",
abstract = "Both natural estrogens and synthetic compounds that mimic estrogen can reach the aquatic environment through wastewater discharges. Because nonylphenol (NP), octylphenol (OP), nonylphenol polyethoxylates (NPE), 17β- estradiol (E2), and ethynylestradiol (EE2) have previously been found to be estrogenic and to occur in wastewater effluents, they were the primary analytes for which the method was developed. Water samples were extracted in situ using solid-phase extraction disks. Analytes were separated by high- pressure liquid chromatography and detected by fluorescence or competitive radioimmunoassay (RIA). Method detection limits (MDLs) using HPLC with fluorescence detection were 11, 2, and 52 ng/L of water for NP, OP, and NPE, respectively. The RIA MDLs for E2 and EE2 were 107 and 53 pg/L, respectively. Samples were collected from four municipal wastewater treatment plants in south central Michigan, eight locations on the Trenton Channel of the Detroit River, MI, and five locations in Lake Mead, NV. Concentrations of NP and OP ranged from less than the MDL to 37 and 0.7 μg/L, respectively. NPE concentrations ranged from less than the MDL to 332 μg/L. Concentrations of E2 and EE2 ranged from less than the MDLs to 3.7 and 0.8 ng/L, respectively.",
author = "Snyder, {Shane A} and Keith, {Timothy L.} and Verbrugge, {David A.} and Snyder, {Erin M.} and Gross, {Timothy S.} and Kurunthachalam Kannan and Giesy, {John P.}",
year = "1999",
month = "8",
day = "15",
doi = "10.1021/es981294f",
language = "English (US)",
volume = "33",
pages = "2814--2820",
journal = "Environmental Science & Technology",
issn = "0013-936X",
publisher = "American Chemical Society",
number = "16",

}

TY - JOUR

T1 - Analytical methods for detection of selected estrogenic compounds in aqueous mixtures

AU - Snyder, Shane A

AU - Keith, Timothy L.

AU - Verbrugge, David A.

AU - Snyder, Erin M.

AU - Gross, Timothy S.

AU - Kannan, Kurunthachalam

AU - Giesy, John P.

PY - 1999/8/15

Y1 - 1999/8/15

N2 - Both natural estrogens and synthetic compounds that mimic estrogen can reach the aquatic environment through wastewater discharges. Because nonylphenol (NP), octylphenol (OP), nonylphenol polyethoxylates (NPE), 17β- estradiol (E2), and ethynylestradiol (EE2) have previously been found to be estrogenic and to occur in wastewater effluents, they were the primary analytes for which the method was developed. Water samples were extracted in situ using solid-phase extraction disks. Analytes were separated by high- pressure liquid chromatography and detected by fluorescence or competitive radioimmunoassay (RIA). Method detection limits (MDLs) using HPLC with fluorescence detection were 11, 2, and 52 ng/L of water for NP, OP, and NPE, respectively. The RIA MDLs for E2 and EE2 were 107 and 53 pg/L, respectively. Samples were collected from four municipal wastewater treatment plants in south central Michigan, eight locations on the Trenton Channel of the Detroit River, MI, and five locations in Lake Mead, NV. Concentrations of NP and OP ranged from less than the MDL to 37 and 0.7 μg/L, respectively. NPE concentrations ranged from less than the MDL to 332 μg/L. Concentrations of E2 and EE2 ranged from less than the MDLs to 3.7 and 0.8 ng/L, respectively.

AB - Both natural estrogens and synthetic compounds that mimic estrogen can reach the aquatic environment through wastewater discharges. Because nonylphenol (NP), octylphenol (OP), nonylphenol polyethoxylates (NPE), 17β- estradiol (E2), and ethynylestradiol (EE2) have previously been found to be estrogenic and to occur in wastewater effluents, they were the primary analytes for which the method was developed. Water samples were extracted in situ using solid-phase extraction disks. Analytes were separated by high- pressure liquid chromatography and detected by fluorescence or competitive radioimmunoassay (RIA). Method detection limits (MDLs) using HPLC with fluorescence detection were 11, 2, and 52 ng/L of water for NP, OP, and NPE, respectively. The RIA MDLs for E2 and EE2 were 107 and 53 pg/L, respectively. Samples were collected from four municipal wastewater treatment plants in south central Michigan, eight locations on the Trenton Channel of the Detroit River, MI, and five locations in Lake Mead, NV. Concentrations of NP and OP ranged from less than the MDL to 37 and 0.7 μg/L, respectively. NPE concentrations ranged from less than the MDL to 332 μg/L. Concentrations of E2 and EE2 ranged from less than the MDLs to 3.7 and 0.8 ng/L, respectively.

UR - http://www.scopus.com/inward/record.url?scp=0033566605&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033566605&partnerID=8YFLogxK

U2 - 10.1021/es981294f

DO - 10.1021/es981294f

M3 - Article

AN - SCOPUS:0033566605

VL - 33

SP - 2814

EP - 2820

JO - Environmental Science & Technology

JF - Environmental Science & Technology

SN - 0013-936X

IS - 16

ER -