Analyzing haplodiploid inheritance of insecticide resistance in whitefly biotypes

D. W. Crowder, A. R. Horowitz, Bruce E Tabashnik, T. J. Dennehy, I. Denholm, K. Gorman, Yves Carriere

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

We developed new methods for analyzing inheritance of insecticide resistance in haplodiploid arthropods and applied them to elucidate resistance of the whitefly Bemisia tabaci (Gennadius) to an insect growth regulator, pyriproxyfen. Two invasive biotypes of this devastating crop pest, the B biotype in Arizona and the Q biotype in Israel, have evolved resistance to pyriproxyfen. Here, we incorporated data from laboratory bioassays and crossing procedures exploiting haplodiploidy into statistical and analytical models to estimate the number of loci affecting pyriproxyfen resistance in strains of both biotypes. In tests with models of one to ten loci, the best fit between expected and observed mortality occurred with a two-locus model for the B biotype strain (QC-02) and for one- and two-locus models for the Q biotype strain (Pyri-R). The estimated minimum number of loci affecting resistance was 1.6 for the B biotype strain and 1.0 for the Q biotype strain. The methods used here can be applied to insecticide resistance and other traits in haplodiploid arthropods.

Original languageEnglish (US)
Pages (from-to)307-315
Number of pages9
JournalBulletin of Entomological Research
Volume99
Issue number3
DOIs
StatePublished - Jun 2009

Fingerprint

insecticide resistance
Aleyrodidae
biotypes
inheritance (genetics)
pyriproxyfen
loci
arthropods
plant pests
insect growth regulators
Bemisia tabaci
Israel
bioassays
methodology

Keywords

  • Bemisia tabaci
  • Biotype
  • Haplodiploid
  • Inheritance
  • Pyriproxyfen
  • Statistical genetics

ASJC Scopus subject areas

  • Insect Science
  • Agronomy and Crop Science

Cite this

Analyzing haplodiploid inheritance of insecticide resistance in whitefly biotypes. / Crowder, D. W.; Horowitz, A. R.; Tabashnik, Bruce E; Dennehy, T. J.; Denholm, I.; Gorman, K.; Carriere, Yves.

In: Bulletin of Entomological Research, Vol. 99, No. 3, 06.2009, p. 307-315.

Research output: Contribution to journalArticle

Crowder, D. W. ; Horowitz, A. R. ; Tabashnik, Bruce E ; Dennehy, T. J. ; Denholm, I. ; Gorman, K. ; Carriere, Yves. / Analyzing haplodiploid inheritance of insecticide resistance in whitefly biotypes. In: Bulletin of Entomological Research. 2009 ; Vol. 99, No. 3. pp. 307-315.
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