Analyzing P-bodies and stress granules in saccharomyces cerevisiae

John R Buchan, Tracy Nissan, Roy Parker

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Eukaryotic cells contain at least two types of cytoplasmic RNA-protein (RNP) granules that contain nontranslating mRNAs. One such RNP granule is a P-body, which contains translatlonally inactive mRNAs and proteins involved In mRNA degradation and translation repression. A second such RNP granule is a stress granule which also contains mRNAs, some RNA binding proteins and several translation initiation factors, suggesting these granules contain mRNAs stalled in translation initiation. In this chapter, we describe methods to analyze P-bodies and stress granules in Saccharomyces cerevisiae, including procedures to determine if a protein or mRNA can accumulate in either granule, if an environmental perturbation or mutation affects granule size and number, and granule quantification methods.

Original languageEnglish (US)
Pages (from-to)619-640
Number of pages22
JournalMethods in Enzymology
Volume470
Issue numberC
DOIs
StatePublished - 2010

Fingerprint

Yeast
Saccharomyces cerevisiae
Messenger RNA
RNA
Proteins
Translational Peptide Chain Initiation
Peptide Initiation Factors
RNA-Binding Proteins
RNA Stability
Protein Biosynthesis
Eukaryotic Cells
Mutation
Degradation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Analyzing P-bodies and stress granules in saccharomyces cerevisiae. / Buchan, John R; Nissan, Tracy; Parker, Roy.

In: Methods in Enzymology, Vol. 470, No. C, 2010, p. 619-640.

Research output: Contribution to journalArticle

Buchan, John R ; Nissan, Tracy ; Parker, Roy. / Analyzing P-bodies and stress granules in saccharomyces cerevisiae. In: Methods in Enzymology. 2010 ; Vol. 470, No. C. pp. 619-640.
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