Androgen regulation of the human FERM domain encoding gene EHM2 in a cell model of steroid-induced differentiation

Sanjay Chauhan, Ritu Pandey, Jeffrey F. Way, Thomas C. Sroka, Manolis C. Demetriou, Susan Kunz, Anne E. Cress, David W. Mount, Roger L. Miesfeld

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

We have developed a cell model to investigate steroid control of differentiation using a subline of HT1080 cells (HT-AR1) that have been engineered to express the human androgen receptor. Dihydrotestosterone (DHT) treatment of HT-AR1 cells induced growth arrest and cytoskeletal reorganization that was associated with the expression of fibronectin and the neuroendocrine markers chromogranin A and neuron-specific enolase. Expression profiling analysis identified the human FERM domain-encoding gene EHM2 as uniquely induced in HT-AR1 cells as compared to 16 other FERM domain containing genes. Since FERM domain proteins control cytoskeletal functions in differentiating cells, and the human EHM2 gene has not been characterized, we investigated EHM2 steroid-regulation, genomic organization, and sequence conservation. We found that DHT, but not dexamethasone, induced the expression of a 3.8kb transcript in HT-AR1 cells encoding a 504 amino acid protein, and moreover, that human brain tissue contains a 5.8kb transcript encoding a 913 amino acid isoform. Construction of an unrooted phylogenetic tree using 98 FERM domain proteins revealed that the human EHM2 gene is a member of a distinct subfamily consisting of nine members, all of which contain a highly conserved 325 amino acid FERM domain.

Original languageEnglish (US)
Pages (from-to)421-432
Number of pages12
JournalBiochemical and Biophysical Research Communications
Volume310
Issue number2
DOIs
StatePublished - Oct 17 2003

Keywords

  • Band 4.1
  • Chromogranin
  • Dihydrotestosterone
  • EPB41L4B
  • FERM domain
  • Fibronectin
  • HT1080
  • Neuroendocrine
  • Yurt

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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