Androgen regulation of the human FERM domain encoding gene EHM2 in a cell model of steroid-induced differentiation

Sanjay Chauhan, Ritu Pandey, Jeffrey F. Way, Thomas C. Sroka, Manolis C. Demetriou, Susan Kunz, Anne E Cress, David W. Mount, Roger Miesfeld

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We have developed a cell model to investigate steroid control of differentiation using a subline of HT1080 cells (HT-AR1) that have been engineered to express the human androgen receptor. Dihydrotestosterone (DHT) treatment of HT-AR1 cells induced growth arrest and cytoskeletal reorganization that was associated with the expression of fibronectin and the neuroendocrine markers chromogranin A and neuron-specific enolase. Expression profiling analysis identified the human FERM domain-encoding gene EHM2 as uniquely induced in HT-AR1 cells as compared to 16 other FERM domain containing genes. Since FERM domain proteins control cytoskeletal functions in differentiating cells, and the human EHM2 gene has not been characterized, we investigated EHM2 steroid-regulation, genomic organization, and sequence conservation. We found that DHT, but not dexamethasone, induced the expression of a 3.8kb transcript in HT-AR1 cells encoding a 504 amino acid protein, and moreover, that human brain tissue contains a 5.8kb transcript encoding a 913 amino acid isoform. Construction of an unrooted phylogenetic tree using 98 FERM domain proteins revealed that the human EHM2 gene is a member of a distinct subfamily consisting of nine members, all of which contain a highly conserved 325 amino acid FERM domain.

Original languageEnglish (US)
Pages (from-to)421-432
Number of pages12
JournalBiochemical and Biophysical Research Communications
Volume310
Issue number2
DOIs
StatePublished - Oct 17 2003

Fingerprint

Gene encoding
Androgens
Dihydrotestosterone
Genes
Steroids
Amino Acids
Chromogranin A
Proteins
Phosphopyruvate Hydratase
Fibronectins
Dexamethasone
Conservation
Brain
Protein Isoforms
Tissue
Cytoskeletal Proteins
Growth

Keywords

  • Band 4.1
  • Chromogranin
  • Dihydrotestosterone
  • EPB41L4B
  • FERM domain
  • Fibronectin
  • HT1080
  • Neuroendocrine
  • Yurt

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Androgen regulation of the human FERM domain encoding gene EHM2 in a cell model of steroid-induced differentiation. / Chauhan, Sanjay; Pandey, Ritu; Way, Jeffrey F.; Sroka, Thomas C.; Demetriou, Manolis C.; Kunz, Susan; Cress, Anne E; Mount, David W.; Miesfeld, Roger.

In: Biochemical and Biophysical Research Communications, Vol. 310, No. 2, 17.10.2003, p. 421-432.

Research output: Contribution to journalArticle

Chauhan, Sanjay ; Pandey, Ritu ; Way, Jeffrey F. ; Sroka, Thomas C. ; Demetriou, Manolis C. ; Kunz, Susan ; Cress, Anne E ; Mount, David W. ; Miesfeld, Roger. / Androgen regulation of the human FERM domain encoding gene EHM2 in a cell model of steroid-induced differentiation. In: Biochemical and Biophysical Research Communications. 2003 ; Vol. 310, No. 2. pp. 421-432.
@article{01edc0c047ba4b2d9e947ccbb6dab398,
title = "Androgen regulation of the human FERM domain encoding gene EHM2 in a cell model of steroid-induced differentiation",
abstract = "We have developed a cell model to investigate steroid control of differentiation using a subline of HT1080 cells (HT-AR1) that have been engineered to express the human androgen receptor. Dihydrotestosterone (DHT) treatment of HT-AR1 cells induced growth arrest and cytoskeletal reorganization that was associated with the expression of fibronectin and the neuroendocrine markers chromogranin A and neuron-specific enolase. Expression profiling analysis identified the human FERM domain-encoding gene EHM2 as uniquely induced in HT-AR1 cells as compared to 16 other FERM domain containing genes. Since FERM domain proteins control cytoskeletal functions in differentiating cells, and the human EHM2 gene has not been characterized, we investigated EHM2 steroid-regulation, genomic organization, and sequence conservation. We found that DHT, but not dexamethasone, induced the expression of a 3.8kb transcript in HT-AR1 cells encoding a 504 amino acid protein, and moreover, that human brain tissue contains a 5.8kb transcript encoding a 913 amino acid isoform. Construction of an unrooted phylogenetic tree using 98 FERM domain proteins revealed that the human EHM2 gene is a member of a distinct subfamily consisting of nine members, all of which contain a highly conserved 325 amino acid FERM domain.",
keywords = "Band 4.1, Chromogranin, Dihydrotestosterone, EPB41L4B, FERM domain, Fibronectin, HT1080, Neuroendocrine, Yurt",
author = "Sanjay Chauhan and Ritu Pandey and Way, {Jeffrey F.} and Sroka, {Thomas C.} and Demetriou, {Manolis C.} and Susan Kunz and Cress, {Anne E} and Mount, {David W.} and Roger Miesfeld",
year = "2003",
month = "10",
day = "17",
doi = "10.1016/j.bbrc.2003.08.147",
language = "English (US)",
volume = "310",
pages = "421--432",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Androgen regulation of the human FERM domain encoding gene EHM2 in a cell model of steroid-induced differentiation

AU - Chauhan, Sanjay

AU - Pandey, Ritu

AU - Way, Jeffrey F.

AU - Sroka, Thomas C.

AU - Demetriou, Manolis C.

AU - Kunz, Susan

AU - Cress, Anne E

AU - Mount, David W.

AU - Miesfeld, Roger

PY - 2003/10/17

Y1 - 2003/10/17

N2 - We have developed a cell model to investigate steroid control of differentiation using a subline of HT1080 cells (HT-AR1) that have been engineered to express the human androgen receptor. Dihydrotestosterone (DHT) treatment of HT-AR1 cells induced growth arrest and cytoskeletal reorganization that was associated with the expression of fibronectin and the neuroendocrine markers chromogranin A and neuron-specific enolase. Expression profiling analysis identified the human FERM domain-encoding gene EHM2 as uniquely induced in HT-AR1 cells as compared to 16 other FERM domain containing genes. Since FERM domain proteins control cytoskeletal functions in differentiating cells, and the human EHM2 gene has not been characterized, we investigated EHM2 steroid-regulation, genomic organization, and sequence conservation. We found that DHT, but not dexamethasone, induced the expression of a 3.8kb transcript in HT-AR1 cells encoding a 504 amino acid protein, and moreover, that human brain tissue contains a 5.8kb transcript encoding a 913 amino acid isoform. Construction of an unrooted phylogenetic tree using 98 FERM domain proteins revealed that the human EHM2 gene is a member of a distinct subfamily consisting of nine members, all of which contain a highly conserved 325 amino acid FERM domain.

AB - We have developed a cell model to investigate steroid control of differentiation using a subline of HT1080 cells (HT-AR1) that have been engineered to express the human androgen receptor. Dihydrotestosterone (DHT) treatment of HT-AR1 cells induced growth arrest and cytoskeletal reorganization that was associated with the expression of fibronectin and the neuroendocrine markers chromogranin A and neuron-specific enolase. Expression profiling analysis identified the human FERM domain-encoding gene EHM2 as uniquely induced in HT-AR1 cells as compared to 16 other FERM domain containing genes. Since FERM domain proteins control cytoskeletal functions in differentiating cells, and the human EHM2 gene has not been characterized, we investigated EHM2 steroid-regulation, genomic organization, and sequence conservation. We found that DHT, but not dexamethasone, induced the expression of a 3.8kb transcript in HT-AR1 cells encoding a 504 amino acid protein, and moreover, that human brain tissue contains a 5.8kb transcript encoding a 913 amino acid isoform. Construction of an unrooted phylogenetic tree using 98 FERM domain proteins revealed that the human EHM2 gene is a member of a distinct subfamily consisting of nine members, all of which contain a highly conserved 325 amino acid FERM domain.

KW - Band 4.1

KW - Chromogranin

KW - Dihydrotestosterone

KW - EPB41L4B

KW - FERM domain

KW - Fibronectin

KW - HT1080

KW - Neuroendocrine

KW - Yurt

UR - http://www.scopus.com/inward/record.url?scp=0141682080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0141682080&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2003.08.147

DO - 10.1016/j.bbrc.2003.08.147

M3 - Article

C2 - 14521927

AN - SCOPUS:0141682080

VL - 310

SP - 421

EP - 432

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 2

ER -