Anesthetic effects on [H+]a and muscle metabolites at rest and following exercise

Ralph F Fregosi, Jerome A. Dempsey

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We determined the effects of ether, intra-arterial Na+ pentobarbital (SP) and decapitaion on arterial [H+] and labile metabolites in plantaris, diaphragm and intercostal muscles of rats at rest and following exhaustive treadmill exercise. 30-60 sec post-analgesia arterial [Lactate] ([LA]a) increased with both anesthetics. SP rats retained CO2 resulting in mixed acidosis, while ether anesthetized rats hyperventilated and maintained pH. During recovery from exercise ether anesthesia had no effect but SP anesthesia caused CO2 retention. Decenpitation of resting rats markedly decreased [CP] and elevated [LA] and [G6P] in all three muscles, thereby negating any subsequent exercise effects. The effects of ether differed from those of SP in that with ether anesthesia: (1) [CP]/[Total Creatine] dell and [LA] rose significantly with exercise; (2) resting [LA] was lower and increased with exercise; and (3) metabolite variability was less with ether than with SP. We conclude that: (a) anesthesia obscured the true effects of exercise on acid-base status by increasing [LA]a in the resting state; (b) decapitation is unsuitable for the study of exercise effects on most muscle metabolites; (c) ether anesthesia is most suitable for use in studies aimed at detecting exercise effects on muscle metabolites and for preserving arterial acid-base status closest to the unanesthetized state.

Original languageEnglish (US)
Pages (from-to)85-98
Number of pages14
JournalRespiration Physiology
Volume65
Issue number1
DOIs
StatePublished - 1986
Externally publishedYes

Fingerprint

Ether
Anesthetics
Muscles
Lactic Acid
Anesthesia
Intercostal Muscles
Decapitation
Acids
Creatine
Pentobarbital
Acidosis
Diaphragm
Analgesia
Skeletal Muscle

Keywords

  • Diaphragm
  • Ether
  • Glycogen
  • High energy phosphates
  • Intercostal
  • Lactate
  • Pentobarbital
  • Plantaris
  • Rat

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Anesthetic effects on [H+]a and muscle metabolites at rest and following exercise. / Fregosi, Ralph F; Dempsey, Jerome A.

In: Respiration Physiology, Vol. 65, No. 1, 1986, p. 85-98.

Research output: Contribution to journalArticle

@article{83a522902e2a45238d350bb791f88c99,
title = "Anesthetic effects on [H+]a and muscle metabolites at rest and following exercise",
abstract = "We determined the effects of ether, intra-arterial Na+ pentobarbital (SP) and decapitaion on arterial [H+] and labile metabolites in plantaris, diaphragm and intercostal muscles of rats at rest and following exhaustive treadmill exercise. 30-60 sec post-analgesia arterial [Lactate] ([LA]a) increased with both anesthetics. SP rats retained CO2 resulting in mixed acidosis, while ether anesthetized rats hyperventilated and maintained pH. During recovery from exercise ether anesthesia had no effect but SP anesthesia caused CO2 retention. Decenpitation of resting rats markedly decreased [CP] and elevated [LA] and [G6P] in all three muscles, thereby negating any subsequent exercise effects. The effects of ether differed from those of SP in that with ether anesthesia: (1) [CP]/[Total Creatine] dell and [LA] rose significantly with exercise; (2) resting [LA] was lower and increased with exercise; and (3) metabolite variability was less with ether than with SP. We conclude that: (a) anesthesia obscured the true effects of exercise on acid-base status by increasing [LA]a in the resting state; (b) decapitation is unsuitable for the study of exercise effects on most muscle metabolites; (c) ether anesthesia is most suitable for use in studies aimed at detecting exercise effects on muscle metabolites and for preserving arterial acid-base status closest to the unanesthetized state.",
keywords = "Diaphragm, Ether, Glycogen, High energy phosphates, Intercostal, Lactate, Pentobarbital, Plantaris, Rat",
author = "Fregosi, {Ralph F} and Dempsey, {Jerome A.}",
year = "1986",
doi = "10.1016/0034-5687(86)90008-3",
language = "English (US)",
volume = "65",
pages = "85--98",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Anesthetic effects on [H+]a and muscle metabolites at rest and following exercise

AU - Fregosi, Ralph F

AU - Dempsey, Jerome A.

PY - 1986

Y1 - 1986

N2 - We determined the effects of ether, intra-arterial Na+ pentobarbital (SP) and decapitaion on arterial [H+] and labile metabolites in plantaris, diaphragm and intercostal muscles of rats at rest and following exhaustive treadmill exercise. 30-60 sec post-analgesia arterial [Lactate] ([LA]a) increased with both anesthetics. SP rats retained CO2 resulting in mixed acidosis, while ether anesthetized rats hyperventilated and maintained pH. During recovery from exercise ether anesthesia had no effect but SP anesthesia caused CO2 retention. Decenpitation of resting rats markedly decreased [CP] and elevated [LA] and [G6P] in all three muscles, thereby negating any subsequent exercise effects. The effects of ether differed from those of SP in that with ether anesthesia: (1) [CP]/[Total Creatine] dell and [LA] rose significantly with exercise; (2) resting [LA] was lower and increased with exercise; and (3) metabolite variability was less with ether than with SP. We conclude that: (a) anesthesia obscured the true effects of exercise on acid-base status by increasing [LA]a in the resting state; (b) decapitation is unsuitable for the study of exercise effects on most muscle metabolites; (c) ether anesthesia is most suitable for use in studies aimed at detecting exercise effects on muscle metabolites and for preserving arterial acid-base status closest to the unanesthetized state.

AB - We determined the effects of ether, intra-arterial Na+ pentobarbital (SP) and decapitaion on arterial [H+] and labile metabolites in plantaris, diaphragm and intercostal muscles of rats at rest and following exhaustive treadmill exercise. 30-60 sec post-analgesia arterial [Lactate] ([LA]a) increased with both anesthetics. SP rats retained CO2 resulting in mixed acidosis, while ether anesthetized rats hyperventilated and maintained pH. During recovery from exercise ether anesthesia had no effect but SP anesthesia caused CO2 retention. Decenpitation of resting rats markedly decreased [CP] and elevated [LA] and [G6P] in all three muscles, thereby negating any subsequent exercise effects. The effects of ether differed from those of SP in that with ether anesthesia: (1) [CP]/[Total Creatine] dell and [LA] rose significantly with exercise; (2) resting [LA] was lower and increased with exercise; and (3) metabolite variability was less with ether than with SP. We conclude that: (a) anesthesia obscured the true effects of exercise on acid-base status by increasing [LA]a in the resting state; (b) decapitation is unsuitable for the study of exercise effects on most muscle metabolites; (c) ether anesthesia is most suitable for use in studies aimed at detecting exercise effects on muscle metabolites and for preserving arterial acid-base status closest to the unanesthetized state.

KW - Diaphragm

KW - Ether

KW - Glycogen

KW - High energy phosphates

KW - Intercostal

KW - Lactate

KW - Pentobarbital

KW - Plantaris

KW - Rat

UR - http://www.scopus.com/inward/record.url?scp=0022443582&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022443582&partnerID=8YFLogxK

U2 - 10.1016/0034-5687(86)90008-3

DO - 10.1016/0034-5687(86)90008-3

M3 - Article

C2 - 3749642

AN - SCOPUS:0022443582

VL - 65

SP - 85

EP - 98

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 1

ER -