Angiopoietin-2 in experimental colitis

Vijay C. Ganta, Walter Cromer, Ginny L. Mills, James Traylor, Merilyn Jennings, Sarah Daley, Benjamin Clark, J. Michael Mathis, Michael J Bernas, Moheb Boktor, Paul Jordan, Marlys H Witte, J. Steven Alexander

Research output: Contribution to journalArticle

55 Scopus citations


Background: The pathophysiology of inflammatory bowel disease (IBD) includes leukocyte infiltration, blood and lymphatic remodeling, weight loss and protein enteropathy. The roles of angiopoietin-2 (Ang-2) in initiating gut inflammation, leukocyte infiltration and angiogenesis are not well understood. Methods: Disease activity index, histopathological scoring, myeloperoxidase assay, immunohistochemistry and sodium dodecyl sulphate-polyacrylamide gel electrophoretic methods were employed in the present study to addess the roles of Ang-2 in experimental colitis. Results: Several important differences were seen in the development of experimental IBD in Ang-2-/- mice. Although weight change and disease activity differ only slightly in WT and Ang-2-/- + DSS treated mice, leukocyte infiltration, inflammation and blood and lymphatic vessel density is significantly attenuated compared to WT + DSS mice. Gut capillary fragility and water export (stool blood and form) appear significantly earlier in Ang-2-/- + DSS mice vs. WT. Colon lengths were also significantly reduced in Ang-2-/- and gut histopathology was less severe in Ang-2-/- compared to WT + DSS. Lastly, the decrease in serum protein content in WT + DSS was less severe in Ang-2-/- + DSS, thus protein losing enteropathy (PLE) a feature of IBD is relieved by Ang-2-/-. Conclusion: These data demonstrate that in DSS colitis, Ang-2 mediates inflammatory hemangiogenesis, lymphangiogenesis and neutrophil infiltration to reduce some, but not all clinical features of IBD. The implications for Ang-2 manipulation in the development of IBD and other inflammatory diseases and treatments involving Ang-2 are discussed.

Original languageEnglish (US)
Pages (from-to)1029-1039
Number of pages11
JournalInflammatory Bowel Diseases
Issue number6
Publication statusPublished - 2010



  • Crohn's disease
  • MECA-32
  • Neutrophils
  • Ulcerative colitis
  • VEGFR-3

ASJC Scopus subject areas

  • Gastroenterology
  • Immunology and Allergy

Cite this

Ganta, V. C., Cromer, W., Mills, G. L., Traylor, J., Jennings, M., Daley, S., ... Alexander, J. S. (2010). Angiopoietin-2 in experimental colitis. Inflammatory Bowel Diseases, 16(6), 1029-1039.