Angiotensin-(1–7) peptide hormone reduces inflammation and pathogen burden during mycoplasma pneumoniae infection in mice

Katie L. Collins, Usir S. Younis, Sasipa Tanyaratsrisakul, Robin Polt, Meredith Hay, Heidi - Mansour, Julie G. Ledford

Research output: Contribution to journalArticlepeer-review

Abstract

The peptide hormone, angiotensin (Ang-(1–7)), produces anti-inflammatory and protective effects by inhibiting production and expression of many cytokines and adhesion molecules that are associated with a cytokine storm. While Ang-(1–7) has been shown to reduce inflammation and airway hyperreactivity in models of asthma, little is known about the effects of Ang-(1–7) during live respiratory infections. Our studies were developed to test if Ang-(1–7) is protective in the lung against overzealous immune responses during an infection with Mycoplasma pneumonia (Mp), a common respiratory pathogen known to provoke exacerbations in asthma and COPD patients. Wild type mice were treated with infectious Mp and a subset of was given either Ang-(1–7) or peptide-free vehicle via oropharyngeal delivery within 2 hours of infection. Markers of inflammation in the lung were assessed within 24 h for each set of animals. During Mycoplasma infection, one high dose of Ang-(1–7) delivered to the lungs reduced neutrophilia and Muc5ac, as well as Tnf-α and chemo-kines (Cxcl1) associated with acute respiratory distress syndrome (ARDS). Despite decreased in-flammation, Ang-(1-7)-treated mice also had significantly lower Mp burden in their lung tissue, indicating decreased airway colonization. Ang-(1–7) also had an impact on RAW 264.7 cells, a com-monly used macrophage cell line, by dose-dependently inhibiting TNF-α production while promoting Mp killing. These new findings provide additional support to the protective role(s) of Ang1-7 in controlling inflammation, which we found to be highly protective against live Mp-induced lung inflammation.

Original languageEnglish (US)
Article number1614
JournalPharmaceutics
Volume13
Issue number10
DOIs
StatePublished - Oct 2021

Keywords

  • Angiotensin-(1–7)
  • Asthma
  • Inflammation
  • Macrophages
  • Mycoplasma pneumoniae

ASJC Scopus subject areas

  • Pharmaceutical Science

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