Angular anisotropy of 2H NMR spectral densities in phospholipid bilayers containing cholesterol

Theodore P. Trouard, Todd M. Alam, Jaroslav Zajicek, Michael F. Brown

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Spin-lattice (R1Z) and quadrupolar order (R1Q) relaxation rates were measured for bilayers of macroscopically oriented 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC-d54) containing cholesterol (1:1 molar ratio), using inversion recovery and broadband Jeener-Broekaert pulse sequences, respectively. Anisotropic deuterium (2H) NMR spin relaxation data were obtained for the first time along the entire flexible acyl chains of the phospholipid molecules in the liquid-crystalline state. Individual spectral densities J10) and J2(2ω0) were calculated from these relaxation rates, and a strong dependence on the angle θ between the macroscopic bilayer normal and the static magnetic field was observed. The spectral densities exhibited opposite angular anisotropies, which were explained in terms of a simple rotational diffusion model for the molecular dynamics of membrane lipid constituents.

Original languageEnglish (US)
Pages (from-to)67-75
Number of pages9
JournalChemical Physics Letters
Volume189
Issue number1
DOIs
StatePublished - Jan 24 1992

Fingerprint

Spectral density
cholesterol
Phospholipids
Anisotropy
Cholesterol
Nuclear magnetic resonance
Dimyristoylphosphatidylcholine
nuclear magnetic resonance
anisotropy
Phosphorylcholine
Deuterium
Membrane Lipids
Molecular dynamics
Magnetic fields
Crystalline materials
Recovery
Molecules
lipids
deuterium
Liquids

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Spectroscopy
  • Condensed Matter Physics
  • Atomic and Molecular Physics, and Optics
  • Surfaces and Interfaces

Cite this

Angular anisotropy of 2H NMR spectral densities in phospholipid bilayers containing cholesterol. / Trouard, Theodore P.; Alam, Todd M.; Zajicek, Jaroslav; Brown, Michael F.

In: Chemical Physics Letters, Vol. 189, No. 1, 24.01.1992, p. 67-75.

Research output: Contribution to journalArticle

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