Angular anisotropy of 2H NMR spectral densities in phospholipid bilayers containing cholesterol

Theodore P. Trouard, Todd M. Alam, Jaroslav Zajicek, Michael F. Brown

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Spin-lattice (R1Z) and quadrupolar order (R1Q) relaxation rates were measured for bilayers of macroscopically oriented 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC-d54) containing cholesterol (1:1 molar ratio), using inversion recovery and broadband Jeener-Broekaert pulse sequences, respectively. Anisotropic deuterium (2H) NMR spin relaxation data were obtained for the first time along the entire flexible acyl chains of the phospholipid molecules in the liquid-crystalline state. Individual spectral densities J10) and J2(2ω0) were calculated from these relaxation rates, and a strong dependence on the angle θ between the macroscopic bilayer normal and the static magnetic field was observed. The spectral densities exhibited opposite angular anisotropies, which were explained in terms of a simple rotational diffusion model for the molecular dynamics of membrane lipid constituents.

Original languageEnglish (US)
Pages (from-to)67-75
Number of pages9
JournalChemical Physics Letters
Volume189
Issue number1
DOIs
StatePublished - Jan 24 1992

ASJC Scopus subject areas

  • Physics and Astronomy(all)
  • Physical and Theoretical Chemistry

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