Angular photodiode array-based device to detect bacterial pathogens in a wound model

Robin E. Sweeney, Jeong Yeol Yoon

Research output: Research - peer-reviewArticle

Abstract

We have developed a device that is able to rapidly and specifically diagnose bacterial pathogens in a wound model based on Mie scatter spectra from a tissue surface. The Mie scatter spectra collected is defined as the intensity of Mie scatter over the angle of detection from a tissue surface. A 650 nm LED perpendicular to the surface illuminates a tissue sample (90°) and photodiodes positioned in 10° increments from 10° to 80° of backscatter act as the detectors to collect these Mie scatter spectra. Through principal component analysis of the Mie scatter spectra collected, we have shown significant differences between Mie scatter spectra of tissues with bacterial pathogens versus those without, as well as significant differences between each species of bacteria tested. The device developed has been tested with a porcine dermis wound model, with samples inoculated with one of three bacterial species (Staphylococcus aureus, Escherichia coli, or Salmonella Typhimurium). Such a device could be critical in the monitoring of a wound for infection and rapid, specific diagnosis of a bacterial wound infection, which would significantly reduce the time and cost associated with specific diagnosis of a bacterial wound infection currently.

LanguageEnglish (US)
JournalIEEE Sensors Journal
DOIs
StateAccepted/In press - Sep 13 2017

Fingerprint

Pathogens
Photodiodes
Tissue
pathogens
photodiodes
infectious diseases
Salmonella
Principal component analysis
Escherichia coli
Light emitting diodes
Bacteria
Detectors
Monitoring
Costs
salmonella
staphylococcus
Escherichia
principal components analysis
bacteria
light emitting diodes

Keywords

  • Escherichia coli
  • Mie scatter
  • Staphylococcus aureus
  • wound infection

ASJC Scopus subject areas

  • Instrumentation
  • Electrical and Electronic Engineering

Cite this

Angular photodiode array-based device to detect bacterial pathogens in a wound model. / Sweeney, Robin E.; Yoon, Jeong Yeol.

In: IEEE Sensors Journal, 13.09.2017.

Research output: Research - peer-reviewArticle

@article{aa78ca390a4c43ebb6fbb8390fc00bf9,
title = "Angular photodiode array-based device to detect bacterial pathogens in a wound model",
abstract = "We have developed a device that is able to rapidly and specifically diagnose bacterial pathogens in a wound model based on Mie scatter spectra from a tissue surface. The Mie scatter spectra collected is defined as the intensity of Mie scatter over the angle of detection from a tissue surface. A 650 nm LED perpendicular to the surface illuminates a tissue sample (90°) and photodiodes positioned in 10° increments from 10° to 80° of backscatter act as the detectors to collect these Mie scatter spectra. Through principal component analysis of the Mie scatter spectra collected, we have shown significant differences between Mie scatter spectra of tissues with bacterial pathogens versus those without, as well as significant differences between each species of bacteria tested. The device developed has been tested with a porcine dermis wound model, with samples inoculated with one of three bacterial species (Staphylococcus aureus, Escherichia coli, or Salmonella Typhimurium). Such a device could be critical in the monitoring of a wound for infection and rapid, specific diagnosis of a bacterial wound infection, which would significantly reduce the time and cost associated with specific diagnosis of a bacterial wound infection currently.",
keywords = "Escherichia coli, Mie scatter, Staphylococcus aureus, wound infection",
author = "Sweeney, {Robin E.} and Yoon, {Jeong Yeol}",
year = "2017",
month = "9",
doi = "10.1109/JSEN.2017.2752155",
journal = "IEEE Sensors Journal",
issn = "1530-437X",
publisher = "Institute of Electrical and Electronics Engineers Inc.",

}

TY - JOUR

T1 - Angular photodiode array-based device to detect bacterial pathogens in a wound model

AU - Sweeney,Robin E.

AU - Yoon,Jeong Yeol

PY - 2017/9/13

Y1 - 2017/9/13

N2 - We have developed a device that is able to rapidly and specifically diagnose bacterial pathogens in a wound model based on Mie scatter spectra from a tissue surface. The Mie scatter spectra collected is defined as the intensity of Mie scatter over the angle of detection from a tissue surface. A 650 nm LED perpendicular to the surface illuminates a tissue sample (90°) and photodiodes positioned in 10° increments from 10° to 80° of backscatter act as the detectors to collect these Mie scatter spectra. Through principal component analysis of the Mie scatter spectra collected, we have shown significant differences between Mie scatter spectra of tissues with bacterial pathogens versus those without, as well as significant differences between each species of bacteria tested. The device developed has been tested with a porcine dermis wound model, with samples inoculated with one of three bacterial species (Staphylococcus aureus, Escherichia coli, or Salmonella Typhimurium). Such a device could be critical in the monitoring of a wound for infection and rapid, specific diagnosis of a bacterial wound infection, which would significantly reduce the time and cost associated with specific diagnosis of a bacterial wound infection currently.

AB - We have developed a device that is able to rapidly and specifically diagnose bacterial pathogens in a wound model based on Mie scatter spectra from a tissue surface. The Mie scatter spectra collected is defined as the intensity of Mie scatter over the angle of detection from a tissue surface. A 650 nm LED perpendicular to the surface illuminates a tissue sample (90°) and photodiodes positioned in 10° increments from 10° to 80° of backscatter act as the detectors to collect these Mie scatter spectra. Through principal component analysis of the Mie scatter spectra collected, we have shown significant differences between Mie scatter spectra of tissues with bacterial pathogens versus those without, as well as significant differences between each species of bacteria tested. The device developed has been tested with a porcine dermis wound model, with samples inoculated with one of three bacterial species (Staphylococcus aureus, Escherichia coli, or Salmonella Typhimurium). Such a device could be critical in the monitoring of a wound for infection and rapid, specific diagnosis of a bacterial wound infection, which would significantly reduce the time and cost associated with specific diagnosis of a bacterial wound infection currently.

KW - Escherichia coli

KW - Mie scatter

KW - Staphylococcus aureus

KW - wound infection

UR - http://www.scopus.com/inward/record.url?scp=85030235823&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85030235823&partnerID=8YFLogxK

U2 - 10.1109/JSEN.2017.2752155

DO - 10.1109/JSEN.2017.2752155

M3 - Article

JO - IEEE Sensors Journal

T2 - IEEE Sensors Journal

JF - IEEE Sensors Journal

SN - 1530-437X

ER -