Annexin II expression is reduced or lost in prostate cancer cells and its re-expression inhibits prostate cancer cell migration

Jun Wei Liu, Jian Jun Shen, Angela Tanzillo-Swarts, Bobby Bhatia, Carlos M. Maldonado, Maria D. Person, Serrine Lau, Dean G. Tang

Research output: Contribution to journalArticle

120 Citations (Scopus)

Abstract

While studying Bim, a BH3-only proapoptotic protein, we identified an ∼36kDa protein, which was abundantly expressed in all five strains of primary normal human prostate (NHP) epithelial cells but significantly reduced or lost in seven prostate cancer cell lines. The ∼36kDa protein was subsequently identified as annexin II by proteomic approach and confirmed by Western blotting using an annexin II-specific antibody. Conventional and 2D SDS-PAGE, together with Western blotting, also revealed reduced or lost expression of annexin I in prostate cancer cells. Subcellular localization studies revealed that in NHP cells, annexin II was distributed both in the cytosol and underneath the plasma membrane, but not on the cell surface. Prostate cancer cells showed reduced levels as well as altered expression patterns of annexin II. Since annexins play important roles in maintaining Ca2+ homeostasis and regulating the cytoskeleton and cell motility, we hypothesized that the reduced or lost expression of annexin I/II might promote certain aggressive phenotypes of prostate cancer cells. In subsequent experiments, we indeed observed that restoration of annexin II expression inhibited the migration of the transfected prostate cancer cells without affecting cell proliferation or apoptosis. Hence, our results suggest that annexin II, and, likely, annexin I, may be endogenous suppressors of prostate cancer cell migration and their reduced or lost expression may contribute to prostate cancer development and progression.

Original languageEnglish (US)
Pages (from-to)1475-1485
Number of pages11
JournalOncogene
Volume22
Issue number10
DOIs
StatePublished - Mar 13 2003
Externally publishedYes

Fingerprint

Annexin A2
Cell Movement
Prostatic Neoplasms
Annexin A1
Prostate
Western Blotting
Annexins
Proteins
Electrophoresis, Gel, Two-Dimensional
Cytoskeleton
Cytosol
Proteomics
Polyacrylamide Gel Electrophoresis
Homeostasis
Epithelial Cells
Cell Proliferation
Cell Membrane
Apoptosis
Phenotype
Cell Line

Keywords

  • Annexins
  • Cell migration
  • Progression
  • Prostate cancer
  • Proteomics

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

Cite this

Liu, J. W., Shen, J. J., Tanzillo-Swarts, A., Bhatia, B., Maldonado, C. M., Person, M. D., ... Tang, D. G. (2003). Annexin II expression is reduced or lost in prostate cancer cells and its re-expression inhibits prostate cancer cell migration. Oncogene, 22(10), 1475-1485. https://doi.org/10.1038/sj.onc.1206196

Annexin II expression is reduced or lost in prostate cancer cells and its re-expression inhibits prostate cancer cell migration. / Liu, Jun Wei; Shen, Jian Jun; Tanzillo-Swarts, Angela; Bhatia, Bobby; Maldonado, Carlos M.; Person, Maria D.; Lau, Serrine; Tang, Dean G.

In: Oncogene, Vol. 22, No. 10, 13.03.2003, p. 1475-1485.

Research output: Contribution to journalArticle

Liu, JW, Shen, JJ, Tanzillo-Swarts, A, Bhatia, B, Maldonado, CM, Person, MD, Lau, S & Tang, DG 2003, 'Annexin II expression is reduced or lost in prostate cancer cells and its re-expression inhibits prostate cancer cell migration', Oncogene, vol. 22, no. 10, pp. 1475-1485. https://doi.org/10.1038/sj.onc.1206196
Liu, Jun Wei ; Shen, Jian Jun ; Tanzillo-Swarts, Angela ; Bhatia, Bobby ; Maldonado, Carlos M. ; Person, Maria D. ; Lau, Serrine ; Tang, Dean G. / Annexin II expression is reduced or lost in prostate cancer cells and its re-expression inhibits prostate cancer cell migration. In: Oncogene. 2003 ; Vol. 22, No. 10. pp. 1475-1485.
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