Anti-EGFR antibody conjugated organic-inorganic hybrid lipid nanovesicles selectively target tumor cells

Siu Ling Leung, Zhengbao Zha, Celine Cohn, Zhifei Dai, Xiaoyi Wu

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Chemical conjugation of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR mAbs) to organic-inorganic hybrid liposomal immunocerasomes via maleimide-thiol coupling chemistry is explored as a mechanism for selectively targeting cancer cells. The cellular uptake and internalization of immunocerasomes are investigated in A431 cells that express an abnormally high level of EGFR, DU145 cells that overexpress EGFR, and HL-60 cells that are used as a negative control. The internalization study reveals a strong correlation between the receptor-mediated endocytosis of immunocerasomes and the membrane expression of EGFR. Further, free anti-EGFR mAbs and immunocerasomes conjugated with anti-EGFR mAbs at nanomolar doses display similar anti-proliferative effects on A431 cells. Additionally, serum proteins greatly reduce the cellular uptake of cerasomes that is mediated by non-specific receptors, but have no adverse effects on the specific EGFR-mediated delivery of immunocerasomes to A431 cells.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume121
DOIs
StatePublished - Sep 1 2014

Fingerprint

Monoclonal antibodies
antibodies
Epidermal Growth Factor Receptor
Antibodies
Lipids
lipids
Tumors
Anti-Idiotypic Antibodies
tumors
Monoclonal Antibodies
Cells
cells
Neoplasms
Sulfhydryl Compounds
Blood Proteins
HL-60 Cells
Proteins
Membranes
Endocytosis
conjugation

Keywords

  • Anti-EGFR antibody
  • Cancer cells
  • Organic-inorganic hybrid lipid nanovesicles
  • Selective targeting

ASJC Scopus subject areas

  • Biotechnology
  • Colloid and Surface Chemistry
  • Physical and Theoretical Chemistry
  • Surfaces and Interfaces

Cite this

Anti-EGFR antibody conjugated organic-inorganic hybrid lipid nanovesicles selectively target tumor cells. / Leung, Siu Ling; Zha, Zhengbao; Cohn, Celine; Dai, Zhifei; Wu, Xiaoyi.

In: Colloids and Surfaces B: Biointerfaces, Vol. 121, 01.09.2014, p. 141-149.

Research output: Contribution to journalArticle

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