Anti-EGFR antibody conjugated organic-inorganic hybrid lipid nanovesicles selectively target tumor cells

Siu Ling Leung, Zhengbao Zha, Celine Cohn, Zhifei Dai, Xiaoyi Wu

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

Chemical conjugation of anti-epidermal growth factor receptor monoclonal antibodies (anti-EGFR mAbs) to organic-inorganic hybrid liposomal immunocerasomes via maleimide-thiol coupling chemistry is explored as a mechanism for selectively targeting cancer cells. The cellular uptake and internalization of immunocerasomes are investigated in A431 cells that express an abnormally high level of EGFR, DU145 cells that overexpress EGFR, and HL-60 cells that are used as a negative control. The internalization study reveals a strong correlation between the receptor-mediated endocytosis of immunocerasomes and the membrane expression of EGFR. Further, free anti-EGFR mAbs and immunocerasomes conjugated with anti-EGFR mAbs at nanomolar doses display similar anti-proliferative effects on A431 cells. Additionally, serum proteins greatly reduce the cellular uptake of cerasomes that is mediated by non-specific receptors, but have no adverse effects on the specific EGFR-mediated delivery of immunocerasomes to A431 cells.

Original languageEnglish (US)
Pages (from-to)141-149
Number of pages9
JournalColloids and Surfaces B: Biointerfaces
Volume121
DOIs
StatePublished - Sep 1 2014

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Keywords

  • Anti-EGFR antibody
  • Cancer cells
  • Organic-inorganic hybrid lipid nanovesicles
  • Selective targeting

ASJC Scopus subject areas

  • Biotechnology
  • Surfaces and Interfaces
  • Physical and Theoretical Chemistry
  • Colloid and Surface Chemistry

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