Anti-IL-4 monoclonal antibody and IFN-γ administration retards development of immune dysfunction and cytokine dysregulation during murine AIDS

Y. Wang, S. K. Ardestani, B. Liang, C. Beckham, Ronald R Watson

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Abstract

This study was designed to determine if administration of anti-interleukin-4 (IL-4) monoclonal antibody (mAb), interferon-γ (IFN-γ) and their combination after LP-BM5 retrovirus infection of female C57BL/6 mice would prevent retrovirus-induction of immunosuppression and cytokine dysregulation. Splenic natural killer (NK) cell activity, T- and B-cell proliferation, and T-helper type 1 (Th1) and Th2 cytokine (IL-2, IFN-γ, IL-5 and IL-10) and monokine [IL-6 and tumour necrosis factor-α (TNF-α)] secretions were monitored, as they are usually altered dramatically after murine retrovirus infection. Administration of IFN-γ and anti-IL-4 significantly prevented retrovirus-induced suppression of splenic NK cell activity, and splenic T- and B-cell proliferation. They also significantly slowed retrovirus-induced elevation of Th2 cytokine (IL-5 and IL-10) release and monokine (IL-6 and TNF-α secretion by splenocytes. They prevented the loss of Th1 cytokine (IL-2 and IFN-γ) release by splenocytes, and alleviated splenomegaly and hypergammaglobulinaemia, precursor signs of development of acquired immune deficiency syndrome (AIDS). These findings could provide insight into the roles of immunomodulator in AIDS treatment as well as the mechanisms by which retrovirus infection induces cytokine dysregulation, facilitating immunodeficiencies in AIDS.

Original languageEnglish (US)
Pages (from-to)384-389
Number of pages6
JournalImmunology
Volume83
Issue number3
StatePublished - 1994

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Murine Acquired Immunodeficiency Syndrome
Interleukin-4
Interferons
Retroviridae Infections
Monoclonal Antibodies
Cytokines
Retroviridae
Monokines
Acquired Immunodeficiency Syndrome
Interleukin-5
Interleukin-10
Interleukin-2
Interleukin-6
B-Lymphocytes
Tumor Necrosis Factor-alpha
Cell Proliferation
Hypergammaglobulinemia
Natural Killer T-Cells
Splenomegaly
Immunologic Factors

ASJC Scopus subject areas

  • Immunology

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Anti-IL-4 monoclonal antibody and IFN-γ administration retards development of immune dysfunction and cytokine dysregulation during murine AIDS. / Wang, Y.; Ardestani, S. K.; Liang, B.; Beckham, C.; Watson, Ronald R.

In: Immunology, Vol. 83, No. 3, 1994, p. 384-389.

Research output: Contribution to journalArticle

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