Antibiotic effects on microbial community characteristics in soils under conservation management practices

Irene M. Unger, Keith W. Goyne, Ann C. Kennedy, Robert J. Kremer, Jean E T Mclain, Clinton F. Williams

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Veterinary antibiotics (VAs) administered to livestock are introduced to agroecosystems via land application of manure, posing a potential human and environmental health risk. Recent evidence suggests that agroforestry and grass vegetative filter strips (VFS) may act to mitigate VA transport or enhance VA degradation; however, VAs may adversely affect soil microbial communities within VFS and thus alter the primary functioning of the VFS. The objectives of this research were to investigate potential changes in microbial community structure and function and to quantify the development of antibiotic resistance in VFS and no-till soils exposed to various VA classes and concentrations. Laboratory mesocosms were established using soils collected from no-till cropland and two VFS (grass and agroforestry). Soils were treated with oxytetracycline or lincomycin (5-200 mg kg-1 soil). Individual mesocosms underwent destructive sampling at nine time points during 63 d, and the soils were tested for soil microbial function (C-utilization, dehydrogenase, and fluorescein diacetate hydrolysis assays), community structure (phospholipid fatty acid analysis), and antibiotic resistance. Functional assays associated with all VA treatments showed an initial inhibitory effect, but this trend was generally reversed by the seventh day. Shifts in microbial community structure and increased antibiotic resistance were not observed, suggesting that the soil microbial communities were robust to the effects of oxytetracycline and lincomycin at test concentrations. This work indicates that using VFS to mitigate VA loss from agroecosystems will not diminish important primary functions associated with VFS use in agriculture.

Original languageEnglish (US)
Pages (from-to)100-112
Number of pages13
JournalSoil Science Society of America Journal
Volume77
Issue number1
DOIs
StatePublished - Jan 2013

Fingerprint

filter strips
conservation management
antibiotics
microbial communities
microbial community
management practice
filter
antibiotic resistance
soil
lincomycin
oxytetracycline
community structure
agricultural ecosystem
agroforestry
agroecosystems
no-tillage
grass
assay
strip tillage
grasses

ASJC Scopus subject areas

  • Soil Science

Cite this

Antibiotic effects on microbial community characteristics in soils under conservation management practices. / Unger, Irene M.; Goyne, Keith W.; Kennedy, Ann C.; Kremer, Robert J.; Mclain, Jean E T; Williams, Clinton F.

In: Soil Science Society of America Journal, Vol. 77, No. 1, 01.2013, p. 100-112.

Research output: Contribution to journalArticle

Unger, Irene M. ; Goyne, Keith W. ; Kennedy, Ann C. ; Kremer, Robert J. ; Mclain, Jean E T ; Williams, Clinton F. / Antibiotic effects on microbial community characteristics in soils under conservation management practices. In: Soil Science Society of America Journal. 2013 ; Vol. 77, No. 1. pp. 100-112.
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