Antibodies specific for Ig idiotype, but not isotype, can substitute for antigen to induce IgM secretion by a B cell clone

Gail Abendroth Bishop, Christopher A. Pennell, William Travis, Geoffrey Haughton, Jeffrey A Frelinger

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Cells of the mouse B cell clone, CH12.LX, secrete IgM when cultured with nominal antigen (sheep erythrocytes, SRBC) and mAbs which bind their membrane Ek molecules. To determine whether anti-lg antibodies can substitute for antigen In the Induction of IgM secretion by CH12.LX, the B cells were cultured with antl-Ek mAbs and antl-IgM or antl-idiotype antibodies. Anti-IgM antibodies were capable of cross-linking the membrane IgM of CH12.LX, and Inhibited mitogen-induced differentiation of the B cells. However, anti-IgM could not substitute for SRBC In delivering a major histocompatibility complex-restricted differentlative signal. In contrast, either polyclonal or monoclonal antibodies specific for the CH12.LX Ig idlotype were fully capable of substituting for antigen in the induction of IgM secretion by CH12.LX. The binding of anti-IgM antibodies did not prevent anti-Idiotype antibodies from delivering a differentiative signal. Thus, binding of ligand to different parts of the membrane Ig molecule can result in the delivery of different biological signals to the B cell.

Original languageEnglish (US)
Pages (from-to)285-290
Number of pages6
JournalInternational Immunology
Volume2
Issue number4
DOIs
StatePublished - Apr 1990
Externally publishedYes

Fingerprint

Clone cells
B Cells
Secretion
Antigens
Substitute
Clone
Antibody
Antibodies
Immunoglobulin M
B-Lymphocytes
Clone Cells
Anti-Idiotypic Antibodies
Cells
Membrane
Membranes
Proof by induction
Molecules
Monoclonal antibodies
Monoclonal Antibody
Erythrocyte

Keywords

  • B cell clone
  • Differentiation
  • IgM secretion
  • Signaling

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Public Health, Environmental and Occupational Health
  • Neuropsychology and Physiological Psychology
  • Transplantation
  • Immunology

Cite this

Antibodies specific for Ig idiotype, but not isotype, can substitute for antigen to induce IgM secretion by a B cell clone. / Bishop, Gail Abendroth; Pennell, Christopher A.; Travis, William; Haughton, Geoffrey; Frelinger, Jeffrey A.

In: International Immunology, Vol. 2, No. 4, 04.1990, p. 285-290.

Research output: Contribution to journalArticle

Bishop, Gail Abendroth ; Pennell, Christopher A. ; Travis, William ; Haughton, Geoffrey ; Frelinger, Jeffrey A. / Antibodies specific for Ig idiotype, but not isotype, can substitute for antigen to induce IgM secretion by a B cell clone. In: International Immunology. 1990 ; Vol. 2, No. 4. pp. 285-290.
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