Antigen dose in the human immune response. Dose-response relationships in the human immune response to Keyhole limpet hemocyanin

John E. Curtis, Evan M. Hersh, William T. Butler, Roger D. Rossen

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

The primary immune response to Keyhole limpet hemocyanin (KLH) was studied in 39 normal adults; 8 were immunized with 5,000 μg, 6 with 100 μg, 6 with 10 μg, 10 with 1 μg, and 9 with 0.1 μg. Immunity to KLH was assessed by delayed hypersensitivity skin testing, hemagglutinin determinations, and KLH-stimulated in vitro lymphocyte blastogenesis. Thirty-three of the 36 persons skin-tested had positive delayed hypersensitivity reactions. The magnitude of the skin test reactions was not related to antigen dose. Immunization with 10 μg or more of KLH induced formation of lymphocytes capable of undergoing increased KLH-stimulated blastogenesis. The lymphocytes of the 19 persons immunized with less than 10 μg did not develop increased in vitro blastogenic responses to KLH. All 39 subjects produced KLH hemagglutinins. Maximal hemagglutinin responses were observed after immunization with 100 μg or more of KLH. The formation of both 7S and 19S antibodies was reduced after immunization with doses of KLH less than 100 μg.

Original languageEnglish (US)
Pages (from-to)61-69
Number of pages9
JournalThe Journal of Laboratory and Clinical Medicine
Volume78
Issue number1
StatePublished - Jul 1971

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Fingerprint Dive into the research topics of 'Antigen dose in the human immune response. Dose-response relationships in the human immune response to Keyhole limpet hemocyanin'. Together they form a unique fingerprint.

Cite this