Antigen epitope-expressing cytokines for DNA immunization

Xianghui He, Tom C. Tsang, Tong Zhang, Phoebe Luo, David T. Harris

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

Strategies to enhance the efficacy of DNA vaccination against malignancy remain to be established. In this study, a plasmid expressing a tumor antigen incorporated into the signal peptide of human IL-2 was tested as a DNA vaccine in a murine model system. Results showed that antigen-specific CTL responses were elicited by intramuscular injection of these plasmids. Importantly, compared with a minigene vector expressing the same epitope, the OVA epitope-incorporated, IL-2 expression plasmid vaccination was more effective in protecting mice from OVA-expressing tumor challenge. The improved efficacy appears to result from enhanced antigen presentation as well as the immunostimulatory activity of IL-2. This approach may provide new perspectives in designing cytokine-adjuvant DNA vaccines for clinical applications.

Original languageEnglish (US)
Pages (from-to)1966-1972
Number of pages7
JournalVaccine
Volume23
Issue number16
DOIs
StatePublished - Mar 14 2005

Keywords

  • Antigen Epitope
  • Cytokine
  • DNA vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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