Antigen-liposome modification of target cells as a method to alter their susceptibility to lysis by cytotoxic T lymphocytes

A. H. Hale, M. J. Ruebush, D. S. Lyles, David T. Harris

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

A method of liposome modification of cell surfaces to render unsuitable target cells susceptible to lysis by anti-viral cytotoxic T lymphocytes (CTLs) is described. Liposomes containing the hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of Sendai virus as well as purified H-2K(k) antigens were capable of binding to the surface of H-2-negative cells and rendering those cells susceptible to lysis by B10·A anti-Sendai virus or anti-H 2K(k) CTLs. The absence from the modifying liposomes of the HN or F proteins or H-2K(k) antigens eliminated the ability of the target cells to be recognized and lysed by either effector cell population. Vesicles containing HN, H-2K(k) molecules, and inactive fusion protein (Fo) were not capable of increasing the susceptibility of H-2-negative target cells to lysis. Liposomes containing inactive fusion protein were similarly unable to render H-2-positive target cells susceptible to lysis by anti-Sendai virus CTLs, suggesting that fusion of the liposomes to the cell surface is a prerequisite to lysis. It did not appear that attachment of liposomes to the cell surface was sufficient for generation of susceptible targets, however, because attachment to the cell surface was observed, as long as the HN glycoprotein was present in the liposomes. These results indicate that purified H-2K(k) glycoproteins are target antigens for anti-H-2(k) CTLs and that B10·A amto-Sendai virus CTLs recognize in an H-2-restricted manner the HN F, or both glycoproteins of Sendai virus in the context of the purified H-2K(k) glycoproteins. This technique of liposome modification of cell surfaces has potential applications in the examination of CTL antigen recognition and immunotherapy of many viral and neoplastic diseases.

Original languageEnglish (US)
Pages (from-to)6105-6108
Number of pages4
JournalProceedings of the National Academy of Sciences of the United States of America
Volume77
Issue number10 II
StatePublished - 1980
Externally publishedYes

Fingerprint

Cytotoxic T-Lymphocytes
Liposomes
Antigens
Sendai virus
Hemagglutinins
Neuraminidase
Glycoproteins
HN Protein
H-2 Antigens
Proteins
Viral Tumor Antigens
Virus Diseases
Immunotherapy

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

@article{9ef10a0a91ef45c7a5b61b1c6e96faca,
title = "Antigen-liposome modification of target cells as a method to alter their susceptibility to lysis by cytotoxic T lymphocytes",
abstract = "A method of liposome modification of cell surfaces to render unsuitable target cells susceptible to lysis by anti-viral cytotoxic T lymphocytes (CTLs) is described. Liposomes containing the hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of Sendai virus as well as purified H-2K(k) antigens were capable of binding to the surface of H-2-negative cells and rendering those cells susceptible to lysis by B10·A anti-Sendai virus or anti-H 2K(k) CTLs. The absence from the modifying liposomes of the HN or F proteins or H-2K(k) antigens eliminated the ability of the target cells to be recognized and lysed by either effector cell population. Vesicles containing HN, H-2K(k) molecules, and inactive fusion protein (Fo) were not capable of increasing the susceptibility of H-2-negative target cells to lysis. Liposomes containing inactive fusion protein were similarly unable to render H-2-positive target cells susceptible to lysis by anti-Sendai virus CTLs, suggesting that fusion of the liposomes to the cell surface is a prerequisite to lysis. It did not appear that attachment of liposomes to the cell surface was sufficient for generation of susceptible targets, however, because attachment to the cell surface was observed, as long as the HN glycoprotein was present in the liposomes. These results indicate that purified H-2K(k) glycoproteins are target antigens for anti-H-2(k) CTLs and that B10·A amto-Sendai virus CTLs recognize in an H-2-restricted manner the HN F, or both glycoproteins of Sendai virus in the context of the purified H-2K(k) glycoproteins. This technique of liposome modification of cell surfaces has potential applications in the examination of CTL antigen recognition and immunotherapy of many viral and neoplastic diseases.",
author = "Hale, {A. H.} and Ruebush, {M. J.} and Lyles, {D. S.} and Harris, {David T.}",
year = "1980",
language = "English (US)",
volume = "77",
pages = "6105--6108",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
number = "10 II",

}

TY - JOUR

T1 - Antigen-liposome modification of target cells as a method to alter their susceptibility to lysis by cytotoxic T lymphocytes

AU - Hale, A. H.

AU - Ruebush, M. J.

AU - Lyles, D. S.

AU - Harris, David T.

PY - 1980

Y1 - 1980

N2 - A method of liposome modification of cell surfaces to render unsuitable target cells susceptible to lysis by anti-viral cytotoxic T lymphocytes (CTLs) is described. Liposomes containing the hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of Sendai virus as well as purified H-2K(k) antigens were capable of binding to the surface of H-2-negative cells and rendering those cells susceptible to lysis by B10·A anti-Sendai virus or anti-H 2K(k) CTLs. The absence from the modifying liposomes of the HN or F proteins or H-2K(k) antigens eliminated the ability of the target cells to be recognized and lysed by either effector cell population. Vesicles containing HN, H-2K(k) molecules, and inactive fusion protein (Fo) were not capable of increasing the susceptibility of H-2-negative target cells to lysis. Liposomes containing inactive fusion protein were similarly unable to render H-2-positive target cells susceptible to lysis by anti-Sendai virus CTLs, suggesting that fusion of the liposomes to the cell surface is a prerequisite to lysis. It did not appear that attachment of liposomes to the cell surface was sufficient for generation of susceptible targets, however, because attachment to the cell surface was observed, as long as the HN glycoprotein was present in the liposomes. These results indicate that purified H-2K(k) glycoproteins are target antigens for anti-H-2(k) CTLs and that B10·A amto-Sendai virus CTLs recognize in an H-2-restricted manner the HN F, or both glycoproteins of Sendai virus in the context of the purified H-2K(k) glycoproteins. This technique of liposome modification of cell surfaces has potential applications in the examination of CTL antigen recognition and immunotherapy of many viral and neoplastic diseases.

AB - A method of liposome modification of cell surfaces to render unsuitable target cells susceptible to lysis by anti-viral cytotoxic T lymphocytes (CTLs) is described. Liposomes containing the hemagglutinin-neuraminidase (HN) and fusion (F) glycoproteins of Sendai virus as well as purified H-2K(k) antigens were capable of binding to the surface of H-2-negative cells and rendering those cells susceptible to lysis by B10·A anti-Sendai virus or anti-H 2K(k) CTLs. The absence from the modifying liposomes of the HN or F proteins or H-2K(k) antigens eliminated the ability of the target cells to be recognized and lysed by either effector cell population. Vesicles containing HN, H-2K(k) molecules, and inactive fusion protein (Fo) were not capable of increasing the susceptibility of H-2-negative target cells to lysis. Liposomes containing inactive fusion protein were similarly unable to render H-2-positive target cells susceptible to lysis by anti-Sendai virus CTLs, suggesting that fusion of the liposomes to the cell surface is a prerequisite to lysis. It did not appear that attachment of liposomes to the cell surface was sufficient for generation of susceptible targets, however, because attachment to the cell surface was observed, as long as the HN glycoprotein was present in the liposomes. These results indicate that purified H-2K(k) glycoproteins are target antigens for anti-H-2(k) CTLs and that B10·A amto-Sendai virus CTLs recognize in an H-2-restricted manner the HN F, or both glycoproteins of Sendai virus in the context of the purified H-2K(k) glycoproteins. This technique of liposome modification of cell surfaces has potential applications in the examination of CTL antigen recognition and immunotherapy of many viral and neoplastic diseases.

UR - http://www.scopus.com/inward/record.url?scp=0019131866&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019131866&partnerID=8YFLogxK

M3 - Article

C2 - 6255476

AN - SCOPUS:0019131866

VL - 77

SP - 6105

EP - 6108

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 10 II

ER -