Antigen recognition by H-2-restricted cytolytic T lymphocytes is not mediated by two independent receptors

David T. Harris, H. R. MacDonald, J. C. Cerottini

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Detergent-solubilized murine cytolytic T lymphocytes (CTL) clones were incorporated into Sendai virus-containing synthetic liposomes. When these liposomes were then fused with other CTL clones possessing a different non-crossreacting specificity, the fusion products were observed to lyse target cells recognized by both parental CTL clones. This method was then used with two H-2-restricted CTL clones of different, non-cross-reacting specificities (anti-H-2[b]-H-Y or anti-H-2[d] Moloney leukemia virus). Once again, the fusion products were found to be lytic against both target cells recognized by the parental clones, but in no instance was there any observable lysis of target cells bearing the same nominal antigen in the context of different H-2 molecules. These results provide strong evidence that antigen recognition by H-2-restricted CTL is not mediated by two independent antigen receptors.

Original languageEnglish (US)
Pages (from-to)330-335
Number of pages6
JournalJournal of Experimental Medicine
Volume159
Issue number1
StatePublished - 1984
Externally publishedYes

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H-2 Antigens
Clone Cells
T-Lymphocytes
Liposomes
Moloney murine leukemia virus
Sendai virus
Antigen Receptors
Detergents
Antigens

ASJC Scopus subject areas

  • Immunology

Cite this

Antigen recognition by H-2-restricted cytolytic T lymphocytes is not mediated by two independent receptors. / Harris, David T.; MacDonald, H. R.; Cerottini, J. C.

In: Journal of Experimental Medicine, Vol. 159, No. 1, 1984, p. 330-335.

Research output: Contribution to journalArticle

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