Antigen-specific modulation of an immune response by in vivo administration of soluble MHC class I tetramers

R. Maile, B. Wang, W. Schooler, A. Meyer, E. J. Collins, Jeffrey A Frelinger

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Abstract

Soluble MHC/peptide tetramers that can directly bind the TCR allow the direct visualization and quantitation of Ag-specific T cells in vitro and in vivo. We used HY-Db tetramers to assess the numbers of HY-reactive CD8+ T cells in HYTCR-transgenic mice and in naive, wild-type C57BL/6 (B6) mice. As expected, tetramer staining showed the majority of T cells were male-specific CD8+ T cells in female HY-TCR mice. Staining of B6 mice showed a small population of male-specific CD8+ T cells in female mice. The effect of administration of soluble MHC class I tetramers on CD8+ T cell activation in vivo was unknown. Injection of HY-Db tetramer in vivo effectively primed female mice for a more rapid proliferative response to both HY peptide and male splenocytes. Furthermore, wild-type B6 female mice injected with a single dose of HY-Db tetramer rejected B6 male skin grafts more rapidly than female littermates treated with irrelevant tetramer. In contrast, multiple doses of HY-Db tetramer did not further decrease graft survival. Rather, female B6 mice injected with multiple doses of HY-Db tetramer rejected male skin grafts more slowly than mice primed with a single injection of tetramer or irradiated male spleen cells, suggesting clonal exhaustion or anergy. Our data highlight the ability of soluble MHC tetramers to identify scarce alloreactive T cell populations and the use of such tetramers to directly modulate an Ag-specific T cell response in vivo.

Original languageEnglish (US)
Pages (from-to)3708-3714
Number of pages7
JournalJournal of Immunology
Volume167
Issue number7
StatePublished - Oct 1 2001
Externally publishedYes

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T-Lymphocytes
Antigens
Staining and Labeling
Transplants
Skin
Peptides
Injections
Graft Survival
Transgenic Mice
Population
Spleen

ASJC Scopus subject areas

  • Immunology

Cite this

Antigen-specific modulation of an immune response by in vivo administration of soluble MHC class I tetramers. / Maile, R.; Wang, B.; Schooler, W.; Meyer, A.; Collins, E. J.; Frelinger, Jeffrey A.

In: Journal of Immunology, Vol. 167, No. 7, 01.10.2001, p. 3708-3714.

Research output: Contribution to journalArticle

Maile, R, Wang, B, Schooler, W, Meyer, A, Collins, EJ & Frelinger, JA 2001, 'Antigen-specific modulation of an immune response by in vivo administration of soluble MHC class I tetramers', Journal of Immunology, vol. 167, no. 7, pp. 3708-3714.
Maile, R. ; Wang, B. ; Schooler, W. ; Meyer, A. ; Collins, E. J. ; Frelinger, Jeffrey A. / Antigen-specific modulation of an immune response by in vivo administration of soluble MHC class I tetramers. In: Journal of Immunology. 2001 ; Vol. 167, No. 7. pp. 3708-3714.
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