Antigenic relationships of murine coronaviruses

Analysis using monoclonal antibodies to JHM (MHV-4) virus

John O. Fleming, Stephen A. Stohlman, Richard C. Harmon, Michael McLai, Jeffrey A Frelinger, Leslie P. Weiner

Research output: Contribution to journalArticle

104 Citations (Scopus)

Abstract

Monoclonal antibodies were produced to JHMV-DL, a neurotropic member of the mouse hepatitis virus (MHV) or murine coronavirus group. Of 23 antibodies isolated, 10 were specific for the major envelope glycoprotein, gp180/90, 10 for the nucleocapsid protein, pp60, and 3 for the minor envelope glycoprotein, gp25. Eleven different MHV isolates were used in antibody binding assays to study antigenic relationships among the viruses. Each MHV isolate tested had a unique pattern of antibody binding, indicating that each is a distinct strain. Conservation of JHMV-DL antigenic determinants varied among the three proteins, with pp60 showing intermediate conservation, gp180/90 little conservation, and gp25 marked conservation in the different MHV strains. Monoclonal antibodies to pp60 proved most useful in delineating antigenic relationships among MHV strains. These antigenic groups correlated with pathogenic types, indicating that pp60 may be one of the gene products which mediates the distinct disease patterns manifested by different murine coronaviruses.

Original languageEnglish (US)
Pages (from-to)296-307
Number of pages12
JournalVirology
Volume131
Issue number2
DOIs
StatePublished - 1983
Externally publishedYes

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Murine hepatitis virus
Coronavirus
Monoclonal Antibodies
Viruses
Antibodies
Glycoproteins
Nucleocapsid Proteins
Epitopes
Genes
Proteins

ASJC Scopus subject areas

  • Virology
  • Infectious Diseases

Cite this

Antigenic relationships of murine coronaviruses : Analysis using monoclonal antibodies to JHM (MHV-4) virus. / Fleming, John O.; Stohlman, Stephen A.; Harmon, Richard C.; McLai, Michael; Frelinger, Jeffrey A; Weiner, Leslie P.

In: Virology, Vol. 131, No. 2, 1983, p. 296-307.

Research output: Contribution to journalArticle

Fleming, John O. ; Stohlman, Stephen A. ; Harmon, Richard C. ; McLai, Michael ; Frelinger, Jeffrey A ; Weiner, Leslie P. / Antigenic relationships of murine coronaviruses : Analysis using monoclonal antibodies to JHM (MHV-4) virus. In: Virology. 1983 ; Vol. 131, No. 2. pp. 296-307.
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