Antihistamines reverse blood-ocular barrier breakdown in experimental diabetes

T. M. Hollis, T. W. Gardner, G. J. Vergis, B. J. Kirbo, C. Butler, R. O. Dull, M. J. Campos, N. A. Enea

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Retinal and other tissue histamine synthesis is increased in experimental diabetes; histamine infusion causes blood-ocular barrier breakdown in nondiabetic rats. We have examined the hypothesis that antihistamines prevent blood-ocular barrier breakdown in streptozotocin diabetes using male Sprague-Dawley rats held 28 days. During the last 7 days they were divided into these treatment groups: control (C), untreated diabetic (D), diabetic rats receiving diphenhydramine-HCl (B), diabetic rats receiving ranitidine (R) and diabetic rats receiving diphenhydramine and ranitidine (BR). Vitreous albumin content was measured 6 hr following fluorescein isothiocyanate bovine serum albumin (FITCBSA) injection. Data show that D had a 98.3% increase in vitreous body FITCBSA over C (p < 0.05) while B and R showed respective decreases of 34.9% and 51.4% compared to D, R being significantly lower than D (p < 0.05). BR showed a decrease of 71% (p < 0.05) compared to D, and R and BR groups were not significantly different from C (p < 0.05). Leakage into the vitreous was from the retina, not the ciliary body. These data indicate that 1) experimental diabetes results in elevated blood-ocular barrier permeability, which can be reversed by diphenhydramine-HCl and ranitidine; and 2) histamine H1- and H2-receptor activation and interaction by altered endogenous histamine metabolism may mediate blood-ocular barrier breakdown, implicating a pathogenic role of histamine in diabetic retinopathy.

Original languageEnglish (US)
Pages (from-to)47-49
Number of pages3
JournalJournal of Diabetic Complications
Volume2
Issue number1
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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