Antimicrobial activity of oregano oil against antibiotic-resistant Salmonella enterica on organic leafy greens at varying exposure times and storage temperatures

Katherine Moore-Neibel, Colin Gerber, Jitendra Patel, Mendel Friedman, Divya Jaroni, Sadhana Ravishankar

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The objective of this study was to evaluate the effectiveness of oregano oil on four organic leafy greens (Iceberg and Romaine lettuces and mature and baby spinaches) inoculated with Salmonella Newport as a function of treatment exposure times as well as storage temperatures. Leaf samples were washed, dip inoculated with S. Newport (6-log CFU/ml) and dried. Oregano oil was prepared at 0.1, 0.3, and 0.5% concentrations in sterile phosphate buffered saline (PBS). Inoculated leaves were immersed in the treatment solution for 1 or 2 min, and individually incubated at 4 or 8 °C. Samples were taken at day 0, 1, and 3 for enumeration of survivors. The results showed that oregano oil was effective against S. Newport at all concentrations. S. Newport showed reductions from the PBS control of 0.7-4.8 log CFU/g (Romaine lettuce), 0.8-4.8 log CFU/g (Iceberg lettuce), 0.8-4.9 log CFU/g (mature spinach), and 0.5-4.7 log CFU/g (baby spinach), respectively. The antibacterial activity also increased with exposure time. Leaf samples treated for 2 min generally showed greater reductions (by 1.4-3.2 log CFU/g), than those samples treated for 1 min; however, there was minimal difference in antimicrobial activity among samples stored under refrigeration and abuse temperatures. This study demonstrates the potential of oregano oil to inactivate S. Newport on organic leafy greens.

Original languageEnglish (US)
Pages (from-to)123-129
Number of pages7
JournalFood Microbiology
Volume34
Issue number1
DOIs
StatePublished - May 2013

Fingerprint

Salmonella Newport
Origanum
Salmonella enterica
oregano
green leafy vegetables
Lettuce
storage temperature
exposure duration
Oils
anti-infective agents
antibiotics
Anti-Bacterial Agents
Ice Cover
oils
Temperature
Spinacia oleracea
romaine lettuce
Lactuca sativa var. capitata
spinach
Phosphates

Keywords

  • Antimicrobials
  • Food safety
  • Oregano oil
  • Organic leafy greens
  • Salmonella

ASJC Scopus subject areas

  • Food Science
  • Microbiology

Cite this

Antimicrobial activity of oregano oil against antibiotic-resistant Salmonella enterica on organic leafy greens at varying exposure times and storage temperatures. / Moore-Neibel, Katherine; Gerber, Colin; Patel, Jitendra; Friedman, Mendel; Jaroni, Divya; Ravishankar, Sadhana.

In: Food Microbiology, Vol. 34, No. 1, 05.2013, p. 123-129.

Research output: Contribution to journalArticle

Moore-Neibel, Katherine ; Gerber, Colin ; Patel, Jitendra ; Friedman, Mendel ; Jaroni, Divya ; Ravishankar, Sadhana. / Antimicrobial activity of oregano oil against antibiotic-resistant Salmonella enterica on organic leafy greens at varying exposure times and storage temperatures. In: Food Microbiology. 2013 ; Vol. 34, No. 1. pp. 123-129.
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